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Taq DNA Ligase
Cat. No./Spec.: E1012-A/1,000 U; E1012-B/2,000 U; E1012-C/10,000 U
Concentration: 40 U/µL
Product Description
Taq DNA Ligase is a heat-resistant ligase that catalyzes the
formation of phosphodiester bonds at the 5´ -phosphate and
3´-hydroxyl ends of two adjacent DNA strands. This reaction can
occur only when the two oligonucleotide chains are perfectly paired
with the complementary target DNA and there is no gap between the
two oligonucleotide chains. Therefore, it can be used to detect
single nucleotide variants. Taq DNA ligase uses NAD as a cofactor.
Taq DNA ligase was active in the range of 37-75°C.
Storage Condition & Shelf Life
Store at -20°C. The product is valid for 2 years.
Source
Recombinant E. coli strain containing ligase gene cloned from
Thermus aquaticus HB8.
Unit Definition
1 unit is the amount of enzyme required to bind 50% of 1 µg BstEII
enzyme to the 12-base pair sticky end of λ DNA for 15 minutes at 45
° C at a total reaction volume of 50 µl.
Scope of Application
• Using ligase chain reaction (LCR) and ligase detection reaction
(LDR) to specifically detect alleles
• Incorporating phosphorylated oligonucleotides during primer
extension amplification for mutation detection
