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Super HIFI DNA Polymerase
For research use only
Cat. No./Spec.
Cat. No P1251 P1252
Spec. 100U 500U
Description
Super HIFI DNA Polymerase is a high-fidelity DNA polymerase. Its
fidelity is approximately 50 times higher than that of regular Taq
DNA polymerase, making it suitable for cloning and other
applications that require high fidelity. Compared to other DNA
polymerases, Super HIFI DNA Polymerase is robust, with short
protocol time, tolerance to PCR inhibitors, and high yields with
fewer enzymes. Amplified fragments can be up to 7.5 kb genome and
20 kb lambda DNA in length. The enzyme has 5'→3' polymerase
activity and 3'→5' exonuclease activity, and the amplification
product has blunt ends.
Applications
gene clone; high fidelity PCR
Features
50X high fidelity of Taq
Tolerant to PCR inhibitors
strong amplification capability for long fragments
Unit Definition
One unit is defined as the amount of enzyme required to incorporate
10 nmol of deoxynucleotide into acid-insoluble species over 30
minutes at 72°C using activated salmon sperm DNA as
template/primer.
Storage
Store at -20°C for 2 years.
