DNA Polymerase I
Cat. No./Spec.: E1021-A/500 U, E1021-B/2,500 U
Concentration: 10 U/µL
Product Description
This product is a template-dependent DNA polymerase capable of
accelerating the synthesis of DNA in the 5'→3' direction. It also
possesses 3'→5' exonuclease (proofreading) activity, 5'→3'
exonuclease activity, and RNase H activity.
Storage Condition & Shelf Life
Store at -20°C.
Source
Recombinant E. coli strain containing the cloned gene polA from E.
coli.
Unit Definition
A unit is defined as the amount of enzyme required to catalyze the
incorporation of 10 nmol of deoxynucleotides into a
polynucleotide substrate within 30 minutes at 37°C.
Features
- Incorporation of modified nucleotides (such as biotin,
digoxigenin, or fluorescently labeled nucleotides).
- Compatible with a variety of buffer systems, including buffers
for restriction enzymes, PCR, and RT reactions.
Scope of Application
- Used in conjunction with DNase, for DNA labeling through nick
translation.
- Used in conjunction with RNase H, for the synthesis of the second
strand of cDNA.
