S1 Nuclease
Cat. No./Spec.: E1017-A/10,000 U
Concentration: 100 U/µL
Product Description
S1 nuclease can degrade single-stranded nucleic acids, releasing
5'-phosphorylated mononucleotides or oligonucleotides. Its activity
on DNA is five times that on RNA. S1 nuclease can also cleave
double-stranded DNA (dsDNA) at single-stranded regions caused by
nicks, gaps, mismatches, or loops. S1 nuclease exhibits
3'-phosphomonoesterase activity.
This enzyme is a glycoprotein with a carbohydrate content of 18%.
Storage Condition & Shelf Life
Store at -20°C.
Source
Aspergillus oryzae strain.
Unit Definition
A unit is defined as the amount of enzyme required to produce 1 µg
of acid-soluble deoxyribonucleotides in 1 minute at 37°C. Enzyme
activity is determined in the following mixture: 30 mM sodium
acetate (pH 4.5), 50 mM NaCl, 0.1 mM ZnCl2, 5% (v/v) glycerol, and
800 µg/mL heat-denatured calf thymus DNA.
Scope of Application
- Removal of single-stranded overhangs from DNA fragments
- Localization of S1 transcripts
- Cleavage of hairpin loops
- In conjunction with Exonuclease III, creating unidirectional
deletions within DNA fragments
Inhibition and Inactivation
- Inhibitors: metal chelating agents, PPi (inorganic
pyrophosphate), Pi (inorganic phosphate), 5'-ribonucleotides, and
deoxyribonucleotides.
- Inactivate by heating at 70°C for 10 minutes in the presence of
EDTA.
