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[China]
Trade Verify
Address: Room230/232, Building 1, No.538 YongfengTun, Haidian District, Beijing
Contact name:Steven
Biovantion Inc. |
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INTENDED USE
Anti-HCV ELISA isan enzyme-linked immunosorbent assay (ELISA) for qualitativedetectionof antibodies to hepatitisCvirus inhumanserumor plasma. It is intended for screeningblooddonorsanddiagnosingpatients relatedtoinfectionwithhepatitisCvirus.
| Product details | Description |
| Delivery | Within 48 hours |
| Packaging Specifications | 8 x 12 strips, 96 wells |
| Country Of Origin | China |
| Manufacturer | 18 months |
| Preservation method | 2℃-8℃ |
| Specimen | Whole blood |
| Assification | class1 |
| Type | Elisa Test Kit |
PROCEDURE
Reagentspreparation:
Allow the reagents to reach room temperature (18-30°C). Check theWash buffer concentratefor thepresenceofsaltcrystals. Ifcrystalshaveformed, resolubilizebywarmingat37°Cuntilcrystals dissolve.DilutetheWashbuffer (20X)asindicatedintheinstructionsforwashing.Usedistilledordeionizedwater andonlycleanvesselstodilutethebuffer.AllotherreagentsareREADYTOUSEASSUPPLIED.
Step1 Preparation:Mark threewellsasNegativecontrol (e.g.B1,C1,D1), twowellsasPositive control (e.g.E1,F1)andoneBlank(e.g.A1,neithersamplesnorHRP-Conjugateshouldbe added into theBlankwell). If theresultswill bedeterminedbyusingdualwavelengthplate reader, the requirement for useof Blankwell couldbeomitted.Useonlynumber of strips
Step2 requiredforthetest.
Step3 AddingDiluent:Add100μlofSpecimenDiluent intoeachwellexcept theBlank. AddingSample: Add 10μl of Positivecontrol, Negative control, andSpecimen into their respectivewells except theBlank. Note: Usea separatedisposal pipette tip for each
Step4specimen, NegativeControl, PositiveControl to avoid cross-contamination. Mix by
Step5 tappingtheplategently. Incubating:Covertheplatewiththeplatecoverandincubatefor30minutesat37°C. Washing:At theendof theincubation, removeanddiscardtheplatecover.Washeachwell5 timeswithdilutedWashBuffer.Each timeallowthemicrowells tosoak for30-60seconds
Step6 Afterthefinalwashingcycle,turndowntheplateontoblottingpaperorcleantowel,andtapitto
Step7removeanyremainders.
Step8 AddingHRP-Conjugate:Add100μlHRP-ConjugateintoeachwellexcepttheBlank. Incubating:Covertheplatewiththeplatecoverandincubatefor30minutesat37°C. Washing:At theendof theincubation, removeanddiscardtheplatecover.Washeachwell5 timeswithdilutedWashingbuffer.Eachtimeallowthemicrowellstosoakfor30-60seconds.
