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[China]
Trade Verify
Address: Room230/232, Building 1, No.538 YongfengTun, Haidian District, Beijing
Contact name:Steven
Biovantion Inc. |
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Verified Suppliers
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INTENDED USE
HBsAgELISAisanenzyme-linkedimmunosorbentassay(ELISA) forqualitativedetectionofHBsAginhumanserum orplasma. It isintendedforscreeningofblooddonorsandfordiagnosingofpatientsrelatedtoinfectionwithhepatitis Bvirus.
| Product details | Description |
| Delivery | Within 48 hours |
| Packaging Specifications | 8 x 12 strips, 96 wells |
| Country Of Origin | China |
| Manufacturer | 18 months |
| Preservation method | 2℃-8℃ |
| Specimen | Whole blood |
| Assification | class1 |
| Type | Elisa Test Kit |
PROCEDURE
Reagentspreparation:
Allowthereagentstoreachroomtemperature(18-30°C).ChecktheWashbufferconcentrate for thepresenceofsaltcrystals. Ifcrystalshaveformed, resolubilizebywarmingat37°Cuntilcrystalsdissolve.Dilute theWashbuffer (20X)as indicated in the instructions forwashing.Usedistilledordeionizedwaterandonlyclean vesselstodilutethebuffer.AllotherreagentsareREADYTOUSEASSUPPLIED.
Step1 Preparation:MarkthreewellsasNegativecontrol (e.g.B1,C1,D1), twowellsasPositivecontrol (e.g. E1,F1)andoneBlank(e.g.A1,neithersamplesnorHRP-Conjugateshouldbeadded intotheBlank well). If theresultswillbedeterminedbyusingdualwavelengthplatereader, therequirement foruseof Blankwellcouldbeomitted.Useonlynumberofstripsrequiredforthetest.
Step2 AddingSample:Add50μlofPositivecontrol,Negativecontrol,andSpecimenintotheirrespectivewells except theBlank.Note:Useaseparatedisposalpipettetipforeachspecimen,NegativeControl, PositiveControltoavoidcross-contamination.Mixbytappingtheplategently.
Step3 AddingHRP-Conjugate:Add50μl of HRP-Conjugate intoeachwell except theBlank, andmixby tappingtheplategently.
Step4 Incubating:Covertheplatewiththeplatecoverandincubatefor60minutesat37°C.
Step5 Washing:At theendof theincubation,removeanddiscardtheplatecover.Washeachwell5timeswith dilutedWashingbuffer.Eachtimeallowthemicrowellstosoakfor30-60seconds.Afterthefinalwashing cycle,turndowntheplateontoblottingpaperorcleantowelandtapittoremoveanyremainders.
Step6 Coloring:Add50μlofChromogenAand50μlChromogenBsolutionsintoeachwell includingtheBlank. Incubate the plate at 37°C for 15minutes avoiding light. The enzymatic reaction between the ChromogensolutionsandtheHRP-ConjugateproducesbluecolorinPositivecontrolandHBsAgpositive samplewells.
Step7 StoppingReaction:Usingamultichannelpipetteormanually,add50μlofStopSolutionintoeachwell andmixgently.IntensiveyellowcolordevelopsinPositivecontrolandHBsAgpositivesamplewells.
Step8 MeasuringtheAbsorbance:CalibratetheplatereaderwiththeBlankwellandreadtheabsorbanceat 450nm. Ifadual filter instrument isused, set thereferencewavelengthat630nm.CalculatetheCut-off valueand evaluate the results. (Note: read the absorbancewithin 10minutes after stopping the reaction).
