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3,3',5,5'-Tetramethylbenzidine dihydrochloride , TMB HCL

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Changzhou Boquan Biotechnology Co.,Ltd

3,3',5,5'-Tetramethylbenzidine dihydrochloride , TMB HCL

Country/Region china
City & Province changzhou jiangsu
Categories Oxide
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Product Details

Chemical name: 3,3',5,5'-Tetramethylbenzidine dihydrochloride

Other name: 4,4'-Diamino-3,3',5,5'-tetramethylbiphenyl dihydrochloride

Chemical formula: C16H20N2.2(HCl)

Molecular weight:313.27

Cas64285-73-0

Melt point>300 ºC

Purity: 99%min

Appearance: withe powder

Storage temperature: 2-8°C

 

Application

3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases.

 

 

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