DL-1,4-Dithiothreitol , DTT

Chemical name: DL-1,4-Dithiothreitol 

Other name: Dithiothreitol; threo-1,4-Dimercapto-2,3-butanediol; Cleland's reagent; DTT

Chemical formula: C₄H₁₀O₂S₂

_{Molecular weight:154.25}

_{Cas: 3483-12-3}

_{Melt point:} 38-43 ºC

Purity: 99%min

Appearance: withe powder

Storage temperature: 2-8°C

Application

An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).

Other Notes

Isolation of RNA using guanidinium salts. Inclusion of a reductant enhances denaturation by breaking intramolecular protein disulfide bonds

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