96 Wells/Kit Mycoplasma Gallisepticum MG Avian Influenza Rapid Test Kit For Poultry

Mycoplasma Gallisepticum MG Antibody ELISA Test Kit for Poultry 96 Wells/Kit

1. Usage

This kit is used to detect Mycoplasma Gallisepticum(MG) antibody in chicken serum, to assess antibody condition by Mycoplasma Gallisepticum(MG) vaccine in chicken farm and assist diagnosis of serological infected chicken.

2. Principle

The Mycoplasma Gallisepticum(MG) antibody ELISA kit is based on an indirect enzymatic immunoassay (Indirect ELISA).The antigen is coated on plates. When a sample serum contains specific antibodies against virus, they will bind to the antigen on plates. Wash the unbound antibodies and other components. Then add a specific enzyme conjugate. After incubation and washing, add the TMB substrate. A colorimetric reaction will appear, measured by a spectrophotometer (450 nm).

3. Reagents

4. Materials required but not provided

1) Micropipettors and disposable tips: 0.5μL~10μL,10μL~100μL,100μL~1000μL
2) Disposable tips
3) 37 ℃ Incubator
4) Measuring cylinder: 500 ml
5) 96 wells microplate reader
6) Distilled water or deionied water
7) Bottle or microplate washing machine

5. Sample preparation

Take animal whole blood, make serum according to regular methods, the serum should be clear, have no hemolysis.

6. Preparation of washing buffer

Return washing buffer to room temperature before use, if there is salty crystals, shake to make the crystals dissolve, then use distilled water or deionized water to dilute it at 10 times. The diluted washing buffer can store for 1 week at 4 ℃.

7. Sample dilution

At serum dilution plate, dilute serum at 1:99 with sample dilution (for example: 495μL sample dilution + 5μL serum)
Notice: Negative control and Positive control do not need dilute. Exchange tip after taking sample every time, record the situation of the sample on plate accurately. Shake the sample evenly before adding it.

8. Notes

1) All reagents should be brought to room temperature and shaken well before use, and stored at 2-8°C after use
2) Do not replace the reagents in the kits with different batch numbers. Avoid reagent contamination during use.
3) Substrate and stop solution may irritate skin and eyes, so be careful when using it.
4) Do not expose the substrate to light and avoid contact with antioxidants.
5) After opening, the wells should be protected from moisture or contact with water (put the unused microplate back in the bag and use a dehydrator at 2~8℃)
6) Properly dispose of all waste before dumping to avoid contamination.
7) Follow the directions exactly for best results. All procedures including pipetting, timing and washing must be accurate.
8) The serum dilution plate is for one-time use, please do not use it twice; its maximum volume is 300μL/well.

9. ELISA procedure

1) Take pre-coated microplate (Can unseal for several time use as per sample quantity), add 100μL diluted serum to sample wells, meanwhile set 1 well for Negative control, 2 wells for Positive control separately. Add 100μL Negative/Positive control to its wells. Shake softly, do not overflow, cover and incubate at 37℃ for 30 min.
2) Pour the liquid out of the wells, add 250 μL diluted washing buffer to each well, pour out. Repeat 4-6 times, at last pat to dry on absorbent paper.
3) Add 100μL Enzyme conjugate to each well, shake softly, cover andincubate at 37℃ for 30 min.
4) Repeat the step 2(washing). Remember pat to dry on absorbent paper at last.
5) Add 100μL substrate to each well, mix properly,react for 10 min atdark at37℃ in dark.
6) Add 50μl of stop solution in each well, and measure the result within 10 min.

10. Results

Read the OD value by microplate-reader at wavelength of 450nm (630nm as reference) .
For the test to be valid:
OD value of Negative control (N) <0.2;
meanwhile OD value of Positive control (P)>0.5.
Calculation method:
OD value of samples/ Average OD value of Positive control = S/P value
Result judge:
S/P < 0.2, Negative;
S/P ≥ 0.2, Positive.

Specifications:96 wells/kit.
Expiry date:12 months.
Storage: Storing at 2-8℃, in the dark.

Q1: How long will it take for you to ship?
A1: Usually ships within 7 working days.

Q2: Do you support OEM/ODM?
A2: can be supported. We can customize according to your specific needs and specific quantities.

Q3: How is your factory doing in terms of quality control?
A3: We have ISO9001 certification and ISO13485 certification, so far all the production process, we have standard rules, we comply with the relevant behavior and laws of the government.

Q4: Is after-sales service guaranteed?
A4: We provide professional online technical after-sales service. If the product fails during the experiment, we can provide
one-to-one guidance through telephone, video and other forms.

Q5: What is your minimum order quantity?
A5: 1Kit.

Q6: What is the shipping method?
A6: It can be shipped by express (FEDEX, UPS, DHL, EMS, etc.) or by air and ground.Please confirm with us before placing an order.