0.5ul Avian Marek'S Avian Influenza Test Kit Rapid Antibody ELISA Kit For Poultry 96 Wells/Kit

Avian Marek's Disease Virus Antibody ELISA Kit for Poultry 96 Wells/Kit

1. Usage

This kit is used to detect avian Marek's disease virus (MDV) antibody in chicken serum, evaluate the antibody status of avian Marek's disease vaccine in chicken farms, and assist in the diagnosis of serum-infected chickens.

2. Principle

The Avian Marek's Disease Antibody ELISA Kit is based on an indirect enzyme immunoassay (indirect ELISA). Purified MDV antigen is coated on the plate. For detection, a diluted serum sample is added, and after incubation, if the serum sample contains specific antibodies against MD virus, they will bind to the antigen coated on the plate. Wash unbound antibody and other components. A specific enzyme conjugate is then added, which will bind the antigen-antibody combination on the plate. After incubation and washing, discard unbound enzyme conjugate; add TMB substrate. A colorimetric reaction occurs, stop solution is added, and the OD value is measured with a spectrophotometer (450 nm).

3. Reagents

4. Materials required but not provided

1) Micropipettors and disposable tips: 0.5μL~10μL,10μL~100μL,100μL~1000μL
2) 37 ℃ Incubator
3) Measuring cylinder: 500 ml
4) 96 wells microplate reader
5) Distilled water or deionied water
6) Bottle or microplate washing machine

5. Sample preparation

Take animal whole blood, make serum according to regular methods, the serum should be clear, have no hemolysis.

6. Preparation of washing buffer

Return washing solution to room temperature before use, if there is salty crystals, shake to make the crystals dissolve, then use distilled water or deionized water to dilute it at 10 times. The diluted washing solution can store for 1 week at 4 ℃.

7. Sample dilution

At serum dilution plate, dilute serum at 1:99 with sample dilution (for example: 495μL sample diluent + 5μL serum)
Notice: Negative control and Positive control do not need dilute. Exchange tip after taking sample every time, record the situation of the sample on plate accurately. Shake the sample evenly before adding it.

8. Notes

1) All reagents should be returned to room temperature and shaken well before use, and stored at 2-8°C after use
2) Do not replace reagents in kits with different lot numbers. Avoid reagent contamination during use.
3) Substrates and stop solutions may irritate skin and eyes, so use caution.
4) Do not expose TMB (Substrate B) to light and avoid contact with antioxidants.
5) After opening the hole, care should be taken to prevent the hole from getting wet or in contact with water (put the unused microplate back in the bag and use a dehydrator at 2~8°C)
6) Properly dispose of all waste before dumping to avoid contamination.
7) Follow the instructions exactly for best results. All procedures including pipetting, timing and washing must be accurate.
8) Serum dilution plate is for single use, please do not reuse; its maximum volume is 300μL/well.

9. ELISA procedure

1) Take a pre-coated microplate (which can be opened and used multiple times according to the sample volume), add 100 μL of diluted serum to each well, and set one well for the negative control, positive control and blank control. Add 100 µL of negative/positive controls to their wells and 100 µL of sample diluent only to blank control wells. Shake gently (do not spill) and incubate at 37°C for 30 minutes.
2) Pour out the liquid in the wells, add 300 μL of diluted washing solution to each well, let stand for 1 min, and pour out. Repeat 3 times, then pat dry on absorbent paper.
3) Add 100 μL of Enzyme Conjugate to each well and incubate at 37°C for 30 min.
4) Repeat step 2 (washing). Finally remember to pat dry on absorbent paper.
5) Add 50 μL of Substrate A to each well, then add Substrate B (50 μL), mix well, and react at 37°C for 10 min in the dark.
6) Add 50 μL of stop solution to each well, and measure the result within 10 minutes.

10. Results

Set zero for the blank well, and test the A450nm (630 nm as a reference) value on the microplate-reader. The conditions for the test to be tenable are that the positive control wells’ A450nm value is greater than or equal to 0.6, and the negative control wells’ A450nm value is less than 0.15. If the test is invalid, the operation is in doubt, retest and observe all the reagents carefully.
If the sample’s A450 value is greater than 0.2+ absorbance of negative control mean, it is judged to be positive; and if less than 0.2+ absorbance of negative control mean, negative. If absorbance of negative control mean is less than 0.05, calculate as 0.05

Specifications: 96 wells/kit or 96*2 wells/kit.
Expiry date:12 months.
Storage: Storing at 2-8℃, in the dark.

Q1: How long will it take for you to ship?
A1: Usually ships within 7 working days.

Q2: Do you support OEM/ODM?
A2: can be supported. We can customize according to your specific needs and specific quantities.

Q3: How is your factory doing in terms of quality control?
A3: We have ISO9001 certification and ISO13485 certification, so far all the production process, we have standard rules, we comply with the relevant behavior and laws of the government.

Q4: Is after-sales service guaranteed?
A4: We provide professional online technical after-sales service. If the product fails during the experiment, we can provide
one-to-one guidance through telephone, video and other forms.

Q5: What is your minimum order quantity?
A5: 1Kit.

Q6: What is the shipping method?
A6: It can be shipped by express (FEDEX, UPS, DHL, EMS, etc.) or by air and ground.Please confirm with us before placing an order.