ISO9001 Veterinary Brucellosis Test Kit Bovine Sheep Dogs Antibody

ISO9001 Veterinary Brucellosis Test Kit Bovine Sheep Dogs Antibody

Bovine and Sheep Brucella Antibody Test Strip 25Tests/Kit ISO9001

1. Principle

This test strip is based on fast immuno-chromatography technique to detect Brucella Ab in Pet, bovine, goat and Pig serum, plasma and whole blood qualitatively. When testing, Brucella Ab in sample combine with antigen coated by colloid gold forming complexes, moving forward to the other head. When reaching the T-line, the specific antigen on the membrane capture the complexes and appear a T line, C line appears, means the test is valid. The T line appears, means there is Brucella Ab in the sample.

2. Veterinary Test Kit Specifications: 25 tests/kit

3. Veterinary Test Kit Components

4. Sample preparation

4.1 Whole blood

Collect anticoagulated whole blood, use for that very day;

4.2 Serum

Collect blood, put at 37℃ for 1-2h, take the supernatant, centrifuge at 1500r/min for 10min, separate the serum. The serum can store for 2-3 days at 4℃, for long term storage, store at -20℃ in frozen.

5. Operation procedures

1) Read the manual carefully and return the test card and sample to room temperature before use.

2) Take out test card, use dropper to absorb the sample, drop 1 drop of sample into buffer, mix evenly, then take 2-3 drops into well mark “S”; when the sample quantity is small, can use Micropipette to transfer 5ul sample into well mark “S” first, then add 2-3 drops of buffer to the well.

3) Read the results in 15min at room temperature.

6. Results

Negative(-): no color reaction on Test Line (T Line),only on Control Line(C Line) being seen red color reaction. It means there is no Brucella Ab in sample or the levels is lower than the detection threshold;

Positive(+): Both on T Line and C line being seen red color reaction. It means there is Brucella Ab in sample;

Invalid: No color reaction on C Line. It means the operation is wrong or the test card is invalid.

7. Notice

1) The test card can be used only once at room temperature, do not reuse or use test card out of expiry date.

2) Return all reagents and sample into room temperature before use.

3) Be careful when prepare sample, wear glove and mask.

4) The whole blood need add anticoagulants, not frozen; serum sample can store in frozen, avoid repeated freezing and thawing.

5) This product is only for in vitro rapid diagnostic veterinary use, can not be quantified, do only qualitative testing.

6) The product test results for reference only, for confirmation, please refer to the relevant national standard methods.

Storage and expiry date

Storage: Store at 4-30 ℃ in dark, sealed, dry place, no frozen.

Expiry date: 12 months; date of production is on box.

Clinical features

(1) Pregnant cows often have miscarriage, stillbirth or weak fetuses at 6-8 months of gestation. After miscarriage, they often discharge dirty gray or brown lochia. Some cases have retained placenta, endometritis, and dirty brownish red exudate injected from vagina. Damage to the breast may cause a decline in the amount of milk produced. In the severe cases, the milk may be completely deteriorated, the breast may become hardened, or even lose the ability to produce milk; It is also accompanied by arthritis. Bulls are mainly characterized by testicular and paraorchitis.

(2) Abortion of sheep and sheep can cause arthritis and synovial bursitis. Rams develop orchitis.

(3) The main causes of pigs are abortion, endometritis and infertility, stillbirth and weak offspring. Acute or chronic orchitis or epididymitis occurs in boars.

Characteristics of pathological changes

It can be seen that fetal septicemia changes, inflammatory reactions (exudation, necrosis, suppuration or caseification) of tissues and organs (uterus, breast, placenta, testis and epididymis) and cell proliferation form granuloma (nodules are composed of epithelioid cells and macrophages) to scarring.

Immunoserology

There are many serological techniques that can be used for the diagnosis of brucella, among which the most widely used serum agglutination test and complement binding test can also be used because of their significance in differential diagnosis. Other serum methods such as tiger red plate agglutination test and antiglobulin test can be used as auxiliary diagnosis.

Allergy

Generally, skin allergy occurs about 20-25 days after brucellosis infection. This method is not suitable for early diagnosis, but can be used for mass quarantine.

Microbiology

Samples can be taken from the uterus, vaginal secretions, blood, viscera, stomach contents, liver, spleen, lymph nodes, and blood of the aborted female for microbiological examination (staining microscopy, isolation and culture, animal inoculation).

Q1: How long will it take for you to ship?
A1: Usually ships within 7 working days.

Q2: Do you support OEM/ODM?
A2: can be supported. We can customize according to your specific needs and specific quantities.

Q3: How is your factory doing in terms of quality control?
A3: We have ISO9001 certification and ISO13485 certification, so far all the production process, we have standard rules, we comply with the relevant behavior and laws of the government.

Q4: Is after-sales service guaranteed?
A4: We provide professional online technical after-sales service. If the product fails during the experiment, we can provide
one-to-one guidance through telephone, video and other forms.

Q5: What is your minimum order quantity?
A5: 1Kit.

Q6: What is the shipping method?
A6: It can be shipped by express (FEDEX, UPS, DHL, EMS, etc.) or by air and ground.Please confirm with us before placing an order.