GMP PPR Antibody Test Strip Peste Des Petits Ruminants 25 Tests/Kit

GMP PPR Antibody Test Strip Peste Des Petits Ruminants 25 Tests/Kit

Peste Des Petits Ruminants PPR Antibody Test Strip 25 Tests/Kit GMP

1. Principle

The rapid test is an immunochromatographic test, which uses colloidal gold immunoassay to detect anti-PPR virus antibodies in whole blood and serum. After adding the sample, the sample moves with the colloidal gold-labeled PPR antigen, whether there is PPR antibody in the sample, it will be wine red when combined with the antigen on the T line; if there is no PPR antibody, there is no color reaction.

2. Components

3.Test procedure

1) Collect whole blood, separate serum (centrifuge at 2000-3000 r/min for 5~15min or 4°C overnight for natural separation), or take whole blood containing anticoagulant as a sample. Whole blood without anticoagulant can also be used as a sample, but it must be tested immediately.

2) Open the package, take out the test card, and place it on a clean and flat table.

3) Aspirate the sample with a dropper and slowly drop 3 drops of the sample into the "S" marked hole.

4) 10-20min judgment result, more than 20min is invalid.

4.Results judgment

1)Positive: control line and test line are both seen wine red, the more the antibody exist, the thicker the color appear;

2)Negative:only control line is seen wine red;

3) Invalidation: control line isn’t seen wine red.

5. Consults for diagnosis

1).If not inoculated by the bacterin againstPPR virus:

a. test line don’t appear visible wine red show there are no antibodies against PPR virus in tested samples, if the animal are healthy, it shows there is no PPRV infection;

b. test line appear visible wine red show the animal was infected with wild virus, should be more observed to validate.

2). if have been inoculated by the bacterin againstPPR virus:

a. the color of test line show no clear color, it means the vaccine immune do not produce protected antibody, it has risk of infected wild virus, suggest having vaccine replant.

b. the color of test line is clear, it means the vaccine has produced protected antibody. If appear infected sign, it may appear immune failure, appear infected PPR.

6. Storage and expiry date

Store at 2 - 30ºC and the shelf life is 24 months.

7. Notice

1) Do not use test card out of date and bad package;

2) Do not use pure water, running water and normal saline as negative control;

3) Store at room temperature (< 30℃) is OK.If store in cool, need return to room temperature firstly, then open package, avoid moisture. Use the test card as soon as possible after open the package.

4) Do not touch the white membrane surface in the middle of test card.

5) To ensure the test quality, take fresh, unpolluted sample. If there is a lot of blood lipids, it will affect the result, so after centrifuge, take serum to test, avoid fat. If the blood or serum is sticky or not enough, use normal saline to dilute at same dilution factor to dilute, then to test.

6) If the serum can not be tested immediately, store at 4℃for short time (in 48hours), store at below -20℃for long-tern storage.

7) Whole blood without anticoagulants should be tested immediately; if already exist part of cruor, separate serum to test. The whole blood with anticoagulants should be tested in 24hours, do not frozen. Freezing and thawing causes hemolysis of red blood cells rupture, seriously affect the test results.

8) When dropping sample, keep head of dropper and “S” well at 1cm to ensure adding sample accurately. If too close, can cause small droplet volume caused by drips judgment error or is not allowed to join the sample size, influence the test results.

9) Avoid bubble when adding sample, the sample quantity should be controlled at about 100ul (about 3 drops). Too much sample would not increase sensitivity. In particular, the whole blood of excessive have made erythrocyte sedimentation in "show hole" place, causing membrane chromatography is so deep into the red background, seriously affect the results.

10) The antibody level is only related to darkness of T line, no relation with C line. Only the C line is clean to see, it means the test is valid.

11) Treat the test card and sample at pollutant according to local regulations.

Treatment measures:

At present, there is no specific treatment for this disease, and the use of antibiotics and sulfonamides at the beginning of the disease can be symptomatic treatment and prevention of secondary infection. For animals with strong resistance and tolerance, symptomatic treatment can be taken to control them to reduce mortality.

(1) Iocain Disinfectant: directly used for wiping and disinfecting the blisters and ulcerated parts of sheep body. It is non-irritating and can quickly improve the continuous deterioration of ulcer focus and avoid the continuous impact of inflammatory focus on sheep body.

(2) Mild cases: use Voretite (sodium cephalothiafur for injection) or Puyuning (sodium cephalothiafur hydrochloride injection) in combination with Wenyanqing (Shuanghuanglian oral liquid) and Qimei (Huangmi polysaccharide injection), twice a day, for 3 to 5 days.
The sheep with fever above 40 ℃ were injected with praepin (5% flunicin meglumine injection+injection) at the same time, 2mg/kg body weight, 1-2 times/day, for 2-3d

(3) Cases of severe decrease in food intake: Wenyanqing and Miglycol can be mixed for 2-3 times a day until the symptoms are relieved.

(4) Disinfection in the house: Iodoan (5% povidone iodine) can be diluted 100-150 times, 1-2 times/day; High-efficiency disinfectant Puyijia (2% glutaraldehyde) or Ortovir (5% benzalkonium bromide) diluted 100 times, 1-2 times a day.

(5) Disinfection outside the house: the highly effective disinfectant Puyijia (2% glutaraldehyde) can be diluted by 100 times, 1-2 times a day; Opuvic (10% month-trimethyl chloride as solution) diluted 100-200 times, 1-2 times/d; Otorvir (5% benzalkonium) diluted 100-200 times, 1-2 times per day.

Q1: How long will it take for you to ship?
A1: Usually ships within 7 working days.

Q2: Do you support OEM/ODM?
A2: can be supported. We can customize according to your specific needs and specific quantities.

Q3: How is your factory doing in terms of quality control?
A3: We have ISO9001 certification and ISO13485 certification, so far all the production process, we have standard rules, we comply with the relevant behavior and laws of the government.

Q4: Is after-sales service guaranteed?
A4: We provide professional online technical after-sales service. If the product fails during the experiment, we can provide
one-to-one guidance through telephone, video and other forms.

Q5: What is your minimum order quantity?
A5: 1Kit.

Q6: What is the shipping method?
A6: It can be shipped by express (FEDEX, UPS, DHL, EMS, etc.) or by air and ground.Please confirm with us before placing an order.