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[China]
Trade Verify
Address: No.246 Shidai Yangguang Road, Yuhua District, Changsha City, Hunan Province, CN, 410000
Contact name:Seven Gong
Hunan Honya Biotech Co., Ltd. |
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Description:
The synthesizer is an economical, versatile mid-throughput platform with short cycle times and low reagent consumption. The system is ideal for the synthesis of primers of various lengths, as well as complex modified oligonucleotides and dye-labeled primers and probes. It can flexibly synthesize sequences longer than 120 mer at 25nmol-300umol synthesis scales. Equipped with 12 channels, each channel can be independently controlled to run, and different reaction times can be set individually to ensure the synthesis quality of each primer while synthesizing 12 primers in parallel.
DNA/RNA oligonucleotides produced by Honya Biotech can be used in gene synthesis, PCR/RT-PCR, siRNA/RNAi, DNA/RNA sequencing, immunology and biochemistry, high-throughput screening, natural product biosynthesis, gene construction, inversion Record PCR, hybridization, single nucleotide polymorphism detection, gene diagnosis and treatment research.
Technical Spots:
1 | lightweight and modular design | which is designed easy to add or removing modules. And optimize the configuration in future. |
2 | Compact design | which using an embedded control computer, no more space for computer, shortening half of the length than other similar synthesizer |
3 | Graphical operation mode | In the testing phase and the initial stage of synthesis, the pipeline can be tested automatically, the status of liquids, pipelines, valves, etc. |
4 | Larger synthesis scale | dual-axis motion system reduced the length of the column support plate ,also saves a lot of space to put larger scale columns. And it can synthesize 12 primers at the same time, which is twice as large as the traditional synthesizer |
5 | AMI/MODIFY can be flexibly configured | Base amidite and modified amidite can be set freely, and you can choose different Synthesis programme |
6 | OXI/SUL can be configured separately | OXI and SUL can be configured separately. |
7 | Independent programme of each channel | Each channel can select different sequence files and process parameters, which can set the initial synthesis position of the sequence and Initialization/finalization mode respectively, etc. For each channels programme can be saved, loaded, tested, and statistically analyzed. |
8 | Real-time display of the synthesis process | During the synthesis process, it can be paused manually or automatically, and then the operate the amidite and other reagent. |
Specification:
Channel | 12 | Synthesis type | DNA/RNA.fluorescent dyes/sulfurizing/Probe |
Mode | reagent injection separately | period(20 mer) | 12 primers of 20bp with 2.5h |
Cycled Time | 4-6min | synthesis range | 25nmol-300umol |
Coupling rate | >99% | max length of primer | 120bp |
Bearing method | Synthetic column | wastes | vacuum |
base/reagent pouring mode | reagent injection separately | base/reagent drive way | protected gas press out |
Base cylinder quantities | Standard 12 pcs,20pcs optional | reagent bottle quantities | 7 pcs |
reagent cylinder configuration | single standard. double optional | reagent specifications | 450ml/4L |
synthesis merger base | Automatic merge the base | System | Win7 |
Data import method | Automatic by Excel | reagent generality | general |
Gas needed | Nitrogen or Argon | color | Matte Black |
reagent pipe dimension | OD1/8' and ID/16' | base pipe dimension | OD1/16, ID0.8mm |
outsize | 910*650*540mm | package size | 1200*700*600mm |
1. Dual Axis motion system:
For traditional synthesis pedestal. There’s one line includes 12 base with long motion length and more space. We updated this technology divided one line into 2 lines. Which saves the synthesis motion time, and saves more space in the box to decrease the synthesis time efficiently.
2. Independent Pipeline System:
For each pipeline of amidite and reagents. We have the independent pipe to avoid pollution interaction between the reagents, the advantage is there’s no dead volume. It also can save the time and cost to clean the pipes by ACN. And each channel can choose the different sequence file and parameter and set Initialization/finalization mode freely. The programme for each pipes can be stored, loaded, inspected and make analysis.
Photo Show:
The nucleic acid synthesis is carried out using the solid phase sialic amide triglyceride method, whereby the 3′ end of the DNA is immobilised on a solid phase substrate and nucleotides are added in the 3′ to 5′ direction until the desired DNA fragment is synthesised. This differs from DNA synthesis by the application of DNA polymerase.
The 3′ end of the first base is immobilised on CPG during synthesis, the 5′-OH of the next base is protected with di-p-tolyl trityl, the amino group on the base is protected with benzoic acid and the 3′-OH is then activated with an amino phosphate compound. 1 base of the 5 5′-OH of 1 base and 3′-OH of the next base form a phosphite triglyceride, which is then oxidised with iodine to a phosphate triglyceride, the protectant on the second base 5′-OH is removed by adding dichloroacetic acid DM cycles through the addition of the next base, and after synthesis the protectant on the 5′-OH is removed with a weak acid The fragment is disconnected from the solid resin with concentrated ammonium hydroxide, the protectant is removed from the base with concentrated ammonium hydroxide under heating, the ammonium hydroxide is removed, the fragment is vacuum dried, and the nucleic acid is purified by liquid chromatography or PAGE.
Company Profile:
Honya Biotech Specialized in sales, design, production, installation and after-sales service for the DNA/RNA synthesizer. We are in this fild for more than 10 years; focused on DNA/RNA Synthesizer, Pipetting Work Station, Deprotection Equipment, Amidite Dissolved Equipment, Purification Instrument, Synthesis Columns, Phosphonamidites, Modification Amidite, Synthesis Reagents, various consumables and CPG, to provide you with the fastest and most efficient DNA/RNA synthesis products and services in the world. We can also customize our instruments to meet your requirements, making DNA/RNA synthesis faster and more flexible.
We are constantly improving the performance of our products, perfecting our production process and getting the details right. We not only provide you with high quality products, but also provide you with training and service. We are equipped with professional management staff and excellent technical team and maintenance personnel to provide our customers with perfect after-sales service.
We have 3 production base in China. Beijing/Qingdao/Changsha city.
Beijing production base is the factory for the instrument for primer synthesis
Qingdao Production base is the factory for the chemicals and consumables.
Changsha city is the office for the overseas marketing.
