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Address: Room 305, Building A, No. 179, Guangpu East Road, Huangpu District, Guangzhou, Guangdong
Contact name:
Guangzhou Dongsheng Biotech Co., Ltd |
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Cat. No./Spec.: K011-A/100U; K011-B/500U; K011-C/2000U; K011-D/5000U
Concentration: 5U/µL
Product Description
In the presence of templates and primers, T4 DNA Polymerase catalyzes DNA synthesis in the 5 ́→3 ́ direction. This enzyme also has the activity of 3 ́→5 ́ exonuclease which is stronger than E. coli DNA polymerase I. Unlike DNA polymerase I, T4 DNA Polymerase does not have 5 ́→3 ́ exonuclease activity.
Components
| Component | K011-A (100U) | K011-B (500U) | K011-C (2,000U) | K011-D (5,000U) |
| T4 DNA Polymerase (5U/µL) | 20 µL | 100 µL | 400 µL | 1 mL |
| 10X Blue Buffer | 100 µL | 250 µL | 500 µL | 1.25 mL |
Storage Condition
All reagents should be stored at -20°C. The product is valid for 12 months.
Unit Definition
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C.
Scope of Application
1. Removal of 3’ overhangs to form blunt ends;
