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5ml DNA Fragments Labeling PCR Kits With Dye Lab Research Use

Foregene Co., Ltd.
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Foregene Co., Ltd.

5ml DNA Fragments Labeling PCR Kits With Dye Lab Research Use

Country/Region china
City & Province chengdu sichuan
Categories Dyestuff Intermediates
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Product Details

 

Fast reagent PCR Easy(with dye) PCR Kits efficient for lab research use

 

Product Pictures

 

Product Descprition

 

This product includes Foregene's unique new generation of Taq——Foregene Taq Hero, optimized dNTPs, MgCl2, reaction buffer, PCR reaction enhancer, optimizer and stabilizer. The 2× PCR HeroTM Mix system has higher tolerance to PCR inhibitors than the ordinary PCR Mix system, and can easily cope with PCR amplification of various complex templates. The unique reaction system and high-efficiency Taq Hero make the PCR reaction have higher amplification efficiency, specificity and sensitivity.

The product is pre-mixed with Electrophoresis Loading Buffer, and after PCR is completed, the sample can be directly loaded for electrophoresis; the operation is more convenient and easier.

 

Specifications:

 

DP-20032-B:2× PCR EasyTM Mix(dye) 5ml(1.7ml × 3)

DP-20033-B:2× PCR EasyTM Mix(dye) 10ml(1.7ml × 6)

DP-20033-B:2× PCR EasyTM Mix(dye) 40ml(1.7ml × 24)

 

Product components:


2× PCR EasyTM Mix(dye) include:0.1U Foregene Taq DNA Polymerase/μl,400 μM dNTPs,20 mM Tris-HCl(pH8.8),100 mM KCl, 3 mM MgCl2, other stabilizers and enhancers,DNA Loading Buffer.

 

Features&advantages:

 

The 2× PCR HeroTM Mix system has higher tolerance to PCR inhibitors than the ordinary PCR Mix system, and can easily cope with PCR amplification of various complex templates. The unique reaction system and high-efficiency Taq Hero make the PCR reaction have higher amplification efficiency, specificity and sensitivity.

 

Kit Application:

 

-PCR amplification of DNA fragments

-DNA labeling

-DNA sequencing

-PCR plus A tail

 

Practical examples:

 

1.Reaction System

 

2× PCR EasyTM Mix(dye)10μl
Template DNAxμl
Primer 1(10μM)0.4μl
Primer 2(10μM)0.4μl
ddH2OFill to 20μl
Total volume

20µl

 

2.Reaction conditions

 

TemperatureTimeCycles
94°C3min1
94°C10sec

 

25-40

55°C20sec
72°C2kb/min
72°C5min1

 

Storage and Shelf life:

 

It can be stored for a long time at -20°C; for frequent use, it can be stored at 4°C for a short time.

 

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