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Address: Building D2, block A, Tri-medical innovation center, No.18, North Bayi Road, Yongning Town, Wenjiang District, Chengdu City, Sichuan Province, P.R.China
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Foregene Co., Ltd. |
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700bp DNA Marker using agarose gel PCR kit DNA ladder 700bp for research use molecular biology tests
Product Details
The DNA markers produced by our company are all obtained by enzyme digestion of plasmids. The markers produced by this process have clean background, clear bands, stable quality and can achieve accurate quantification of markers. The product contains two dyes (cyan dye and yellow), and the migration rate of electrophoresis can be judged by the color change during electrophoresis. The migration rate of cyan dye is the same as that of 3-5kb in 1% agarose gel, and the migration rate of yellow dye is about The migration rate is the same as that of the 50bp band, and the electrophoresis progress can be directly observed with the naked eye. It is easy to use and the electrophoresis image is clear.
This 700bp DNA Ladder is a ready-to-use product and already contains 1x Loading Buffer. You can directly take an appropriate amount of Marker for electrophoresis according to experimental needs. DNA Marker Ⅰ consists of 7 DNA bands, the DNA bands are: 100bp (50ng/5μl), 200bp (30ng/5μl), 300bp (30ng/5μl), 400bp (40ng/5μl), 500bp (25ng/5μl) ), 600bp(30ng/5μl), 700bp(35ng/5μl).
Specification
| Catalogue Number | Specifications |
| DMO-001-01 | 250ml×1 tube |
| DMO-001-02 | 1mL×1 tube |
Concentration
The product concentration is about 240ng/5μl
Storage
4℃ after melting, -20℃ for long term storage.
Instructions for use
1. When the width of the sample hole during electrophoresis is less than 6mm, take 5μl of product each time for electrophoresis. If the sample hole is wider, the sample volume can be appropriately increased;
2. The recommended electrophoresis conditions are 2% agarose gel, the voltage is 4-10V/cm, and the electrophoresis bands are observed under UV conditions.
Precautions
1. The purity of Agarose has a great influence on the clarity of DNA bands. Please use high-quality Agarose for electrophoresis.
2. The concentration of agarose gel is closely related to the separation performance of DNA fragments. Please use a gel of appropriate concentration during electrophoresis.
3. Change the electrophoresis buffer in time and use a freshly prepared agarose gel so as not to affect the electrophoresis results.
4. When performing electrophoresis, thoroughly dissolve and mix to avoid repeated freezing and thawing and contamination.
