Host DNA/RNA Removal Kit For Microbial DNA Enrichment

Host DNA/RNA Removal Kit for Microbial DNA Enrichment

Company Information

BunnyTeeth Technology Inc. is committed to building a complete core technology platform for bioengineering, building a high-quality biotech product chain and a technical service chain, with the ambition to become a global leader in molecular and genetic diagnostic. BunnyTeeth Technology Inc. will also provide customers with a full-range of one-step service solution in the field of molecular diagnostics and genetic diagnostic.

Product Information

Host DNA/RNA Removal Kit for Microbial DNA Enrichment

Host DNA/RNA Removal Kit for Microbial DNA Enrichment is suitable for the selective and efficient removal of host cells and nucleic acids from various types of biological fluid samples with high host cells (blood, sputum, saliva, alveolar lavage, etc.), thus achieving enrichment and purification of microorganisms. This kit can significantly reduce the host background and substantially improve the accuracy and sensitivity of mNGS detection so it has good prospects for clinical application.

Product Advantages

Various Sample Types: Widely support blood, saliva, alveolar lavage fluid, sputum, swabs and other biological fluid samples.

Unbiased: Unbiased microbial nucleic acid extraction, compatible with multiple pathogen types, minimizes microbial loss in samples.

High Efficiency and Simplicity: Fast and simple operation to minimize the host background and enrich microorganisms efficiently.

Wide Applications: Compatible with a variety of downstream applications, including PCR, qPCR, mNGS, etc., ensuring the accuracy and precision of downstream experiments.

Experimental Data

High quality of Microbial DNA Enrichment

Figure 1. Efficient host removal.

Use Host DNA/RNA Removal Kit for Microbial DNA Enrichment and Zymo similar product to remove host DNA and then extract microbial DNA from 5 body fluid samples. qPCR was performed on the conserved sequences of host DNA. higher Ct values indicated lower host DNA yield. The host removal ability of Host DNA/RNA Removal Kit for Microbial DNA Enrichment was is the same as similar products from competing companies.

Stable, balanced and accurate sequence information

Figure 3. Excellent QC data for mNGS.

Use Host DNA/RNA Removal Kit for Microbial DNA Enrichment to remove host DNA in alveolar lavage samples. Microbial DNA obtained with BunnyMag Body Fluids Microbiome DNA Isolation Kit entered the mNGS process with excellent quality control performance.

Main Composition

Host Lysis Solution

Nuclease

Nuclease Buffer

Proteinase K

Storage Condition

Please take out Nuclease and store at -20℃, Proteinase K at 2-8℃, and other reagents at room temperature.

The validity period is 12 months.

Operating Process