CFDNA Isolation mNGS Library Preparation Kits Targeted Drug Guidance

Cell-free Fetal DNA Isolation Kit (PCR, mNGS library preparation) for Targeted Drug Guidance

High quality nucleic acid is the guarantee for genomics research. BunnyTeeth Technology uses superparamagnetic bead purification technology to provide users with a highly sensitive nucleic acid extraction solution that easily achieves trace nucleic acid extraction for low concentration. As verified by thousands of clinical samples, Cell-free Fetal DNA Isolation Kit (PCR, mNGS library preparation) for Targeted Drug Guidance can extract high quality cfDNA from cell-free body fluids such as fresh or frozen plasma, serum, pleural fluid, ascites and cerebrospinal fluid for scientific research and clinical in-vitro diagnostic use.

Advantages

1. Efficient Extraction: High DNA extraction yield, good purity, effective removal of impurities and downstream PCR inhibitors.
2. Flexible Sample Incorporation: Easy extraction of cfDNA from 0.5mL to 4mL samples with elution volumes as low as 20μL.
3. Diverse Applications: Validated by clinical samples, designed for tumor, infection and prenatal diagnosis with liquid biopsy.
4. Wide Support of Automated Platform: Compatible with manual and automated extraction procedures, support Tecan, Hamilton, PE and other nucleic acid extraction workstations.

Figure 1. Efficient recovery of plasma cfDNA with different nucleic acid quantity

Addition of a known amount of exogenous DNA fragment to plasma, simulating 3 gradients of high and low nucleic acid content samples, and using 3 kits for simultaneous nucleic acid extraction. Cell-free Tumor DNA Isolation Kit (PCR, ddPCR) for Tumor Mutation Detection can recover cfDNA fragments from samples with different nucleic acid quantity efficiently, and the nucleic acid yield and reproducibility are better than similar products from competing companies.

Figure 2. Detected mutation abundance is in consistent with the theoretical abundance of the reference standard with a ratio of 1

After using Cell-free Fetal DNA Isolation Kit (PCR, mNGS library preparation) for Targeted Drug Guidance to extract standard cfDNA into NGS process, the detected mutation abundance is highly consistent with the theoretical mutation abundance of ref. standard, and the similarity of mutation abundance detection with similar products of competing companies is high.

Main Composition: Lysis Binding Buffer, Washing Buffer 1 & 2, Proteinase K, Superparamagnetic beads

Storage Temperature: 10- 30 ℃ & 2-8 ℃

Shelf life: 12 months