HBcAb Blood Test Elisa Test Enzyme Linked Immunosorbent Assay

HBcAb Elisa Kit Enzyme Linked Immunosorbent Assay Elisa

HBcAb ELISA Kit
Catalog No.: BE105A

1.SUMMARY

1 Hepatitis B is an infectious illness caused by hepatitis B virus (HBV) which is an enveloped, double-stranded DNA virus belonging to the Hepadnaviridae family and is recognized as the major cause of blood transmitted hepatitis together with hepatitis C virus (HCV). HBV is excreted in body fluids such as semen, saliva, blood and urine in persons with acute or chronic infection.

2 The hepatitis B virus is known as a blood-borne virus because it is transmitted from one person to another via blood or fluids contaminated with blood.

3 Transmission of hepatitis B virus results from exposure to infectious blood or body fluids.When HBV invades the body it causes liver damage through induction of auto-immunity.

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• 2. MATERIALS PROVIDED

1.Microplate 1 block (96wells)
2.Negative Control 1 ml ×1
3.Positive Control 1 ml ×1
4.Conjugate 6 ml ×1
5.Substrate Solution A 6 ml ×1
6.Substrate Solution B 6 ml ×1
7.20×Wash Buffer 40 ml ×1
8.Stop Solution 6 ml ×1
9.Plate Cover 3 piece
10.Insert 1 copy

3 MATERIALS REQUIRED BUT NOT PROVIDED

1. Micropipettes: 0.02, 0.05, 0.10, 0.15, 0.20, and 1.0 ml.

2. Disposable pipette tips.

3. Distilled or deionized water.

4. Humidified Box capable of maintaining 37°C

5. Absorbent paper or paper towel.

6. Microtiter plate or strip-well washer

7. Microtiter plate reader with 450nm (or 450 nm/630nm) wavelength

8. Timer

TEST PROCEDURE

1. Bring ELISA Kit (all reagents), and Specimens to room temperature before use (approximately 30 minutes).

2. Check the Wash buffer concentrate for the presence of salt crystals. If crystals have formed in the solution, resolubilize by warming at 37℃ until crystals dissolve. Dilute concentrated wash buffer 1:19 with ddH₂O. Use only clean vessels to dilute the buffer.

3. For each test, set one blank, two positive and two negative controls. Add 50μl Positive control, Negative control, and Specimen into their respective wells. Add 50μl of HRP-Conjugate to each well except the Blank and mix by tapping the plate gently. Neither samples nor HRP-Conjugate should be added into the Blank well.

4. Cover wells with seal paper, and place the microtiter plate into a humidified box and incubate at 37°C for 30 min.

5. Discard the liquid in all wells and fill the wells with wash solution. Lay aside for 15 seconds, discard the liquid in all wells and fill the wells with wash solution. Repeat 5 times and blocking the rim of wells on absorbent paper for a few seconds wells after last wash.

6. Add 50μl substrate A and B respectively to each well including the blank well. Mix gently, protected from light and incubate 15 minutes at 37°C.

7. Add one drop (50 ul) of Stop Solution to each well including blank well to stop the color reaction.

8. Read the OD value at 450 nm/630 nm with dual filter plate reader. It is option to read the OD value at 450 nm with single filter plate reader. (Using the OD value of the blank well to correct all the OD reading from all wells)