Inhibin B ELISA TEST KIT

INTENDED USE

The T-SPOT.TB test is an in vitro diagnostic test for the detection of effector T cells that respond to stimulation by Mycobacterium tuberculosis antigens ESAT-6 and CFP 10 by capturing interferon gamma (IFN-) in the vicinity of T cells in human whole blood collected in sodium citrate or sodium or lithium heparin. It is intended for use as an aid in the diagnosis of M. tuberculosis infection. The T-SPOT.TB test is an indirect test for M. tuberculosis infection (including disease) and is intended for use in conjunction with risk assessment, radiography and other medical and diagnostic evaluations.

Assay procedure:

Step 1: Prepare all reagents, working standards, Blank and samples as directed in the previous sections.

Step 2: Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the kkkkkdesiccant back into the pouch and seal the ziploc, store unused wells at 4°C

Step 3:Pipette standard 50μl to testing standard well,Pipette Sample dilution 40μl to testing sample well, then add kkkkkktesting sample 10μl (sample final dilution is 5-fold), Pipette sample to wells, don’t touch the well wall as far as kkkkkkpossible, and mix gently.

Step 4: Incubate: Cover with the adhesive strip provided, incubate for 30 min at 37℃.

Step 5: Configurate liquid: Dilute wash solution 30-fold with distilled water.

Step 6: Washing: Uncover the adhesive strip, discard liquid, pipette washing buffer to every well, still for 30s then drain, kkkkkrepeat 5 times.

Step 7: Add enzyme: Pipette HRP-Conjugate reagent 50μl to each well, except blank well.

Step 8: Incubate: Operation with 4

Step 9: Washing: Operation with 6.

Step10: Color: Pipette Chromogen Solution A 50ul and Chromogen Solution B 50ul to each well, avoid the light kkkkkpreservation for 15 min at 37℃.

Step 11: Stop the reaction: Pipette Stop Solution 50μl to each well, stop the reaction (the blue change to yellow).

Step 12: Calculate: take blank well as zero. Read absorbance at 450nm after pipette Stop Solution within 15min.