Actinobacillus Pleuropneumoniae Porcine Test Kit Rt Pcr Fluorescence APP

Actinobacillus pleuropneumoniae(APP) Nucleic Acid Detection Kits

(PCR-Fluorescent Probe Method)​

Intended use:

This kit is suitable for the detection of DNA of Actinobacillus pleuropneumoniae (APP) in tracheal secretions swabs, lung tissue samples, etc., for the auxiliary diagnosis of Actinobacillus pleuropneumoniae.

Principle:

This kit choose swine Actinobacillus pleuropneumoniae conservative design specific gene probe, the probe can with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, thereby quenching groups out of the 3' end, Fluorescence was produced and detected by fluorescence PCR instrument to achieve the detection of actinobacillus pleuropneumoniae nucleic acid in the completely closed reaction system.

Main components:

Sample requirements:

1. Sample type:

Swab of tracheal secretion, lung tissue, etc

2. Sample collection and transportation:

Sample collection and transportation shall be carried out in accordance with NY/T 541. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.

3. Sample preservation:

It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -70℃

The results of interpretation:

Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.