This kit is applied for the specific detection of feline
leukemia virus (FeLV) in feline/cat EDTA anticoagulant whole blood
samples.
Principle
This kit uses RNA as a template and primers as a starting point to
synthesize cDNA strands complementary to RNA template under the
action of high-efficiency reverse transcriptase. Choose cat
coronavirus gene conservative design specific probe, the probe can
with primer amplification area in the middle of a specific binding
DNA template, in the process of PCR extension, the circumscribed
Taq polymerase enzymes from the 5 'end of fluorescent groups on the
probe will be cut down, make it free in the reaction system,
thereby quenching groups out of the 3' end, glow, Detection of cat
coronavirus nucleic acid in a completely closed reaction system was
realized by fluorescence PCR instrument.