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FeLV Feline Cat Test Kit EDTA Anticoagulant Feline Leukemia Test Kit PCR

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FeLV Feline Cat Test Kit EDTA Anticoagulant Feline Leukemia Test Kit PCR

City & Province guangzhou
Categories Dyestuff Intermediates
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Product Details

Feline Leukemia virus (FeLV)Nucleic Acid EDTA Anticoagulant RT fluorescence quantitative PCR probe detection kit

 

Intend for Use

This kit is applied for the specific detection of feline leukemia virus (FeLV) in feline/cat EDTA anticoagulant whole blood samples.

 

Principle

This kit uses RNA as a template and primers as a starting point to synthesize cDNA strands complementary to RNA template under the action of high-efficiency reverse transcriptase. Choose cat coronavirus gene conservative design specific probe, the probe can with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, thereby quenching groups out of the 3' end, glow, Detection of cat coronavirus nucleic acid in a completely closed reaction system was realized by fluorescence PCR instrument.
 

 

ComponentSpecification
PCR reaction liquid8 tubes
Positive control1 tube
Negative control1 tube

 

 

Storage: keep away from direct light  uner -20±5℃

 

 

 

 

The results of Analysis 

 

 
ResultsCt Value
PositiveCt≤40
NegativeNo Ct value or Ct value=0
Gray area40<Ct≤45

 

 

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