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Quantitative Feline FIV Test Kit Immunodeficiency Feline Cat Test PCR

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Quantitative Feline FIV Test Kit Immunodeficiency Feline Cat Test PCR

City & Province	guangzhou
Categories	Dyestuff Intermediates

Product Details

Feline immunodeficiency virus (FIV)Nucleic Acid EDTA Anticoagulant RT fluorescence quantitative PCR probe detection kit

Intend for Use

This kit is applied for the specific detection of feline immunodeficiency virus (FIV) in feline/cat EDTA anticoagulant whole blood samples.

Principle

This kit uses RNA as a template and primers as a starting point to synthesize cDNA strands complementary to RNA template under the action of high-efficiency reverse transcriptase. Choose cat coronavirus gene conservative design specific probe, the probe can with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, thereby quenching groups out of the 3' end, glow, Detection of cat/ feline immunodeficiency nucleic acid in a completely closed reaction system was realized by fluorescence PCR instrument.

Component	Specification
PCR reaction liquid	8 tubes
Positive control	1 tube
Negative control	1 tube

Storage: keep away from direct light uner -20±5℃

The results of Analysis

Results	Ct Value
Positive	Ct≤40
Negative	No Ct value or Ct value=0
Gray area	40＜Ct≤45

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Guangzhou BioKey Healthy Technology Co.Ltd

Quantitative Feline FIV Test Kit Immunodeficiency Feline Cat Test PCR

Product Details

Feline immunodeficiency virus (FIV)Nucleic Acid EDTA Anticoagulant RT fluorescence quantitative PCR probe detection kit

Intend for Use

Principle

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