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Trade Verify
Address: Add：No.2, Ruitai Rd, Guangzhou Hi-Tech Industrial Development Zone, 510670, CHINA
Contact name:Lisa
Guangzhou BioKey Healthy Technology Co.Ltd |
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Verified Suppliers
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Intended use:
Principle:
UP+UU product lyophilization pacakage:
Main components:
| NO. | Components | BIK-QL-H0022S |
1 | PCR Enzyme Mix(Lyophilized) | 48 tests/kit |
| 2 | Positive Control | 100 μL/tube |
| 3 | Negative Control | 100 μL/tube |
| 4 | RNase-free water | 1mL/tube |
| 5 | Paraffin oil | 1.5mL/tube |
Specimen requirements:
1. Specimen type
Male urethral secretions or female cervical secretions.
2. Specimen Preservation
The specimen can be stored for 3 months at -20±5℃ and for a long time below -70℃
PCR Amplification condition:
PCR amplification according to the following procedures:
| Step | Cycle number | Temperature | Time |
| 1 | 1 | 95℃ | 3min |
| 2 | 40 | 95℃ | 10s |
| 60℃ | 30s collect fluorescent |
Instrument detection channel selection: FAM and HEX/VIC.
Quality control:
Negative Control: None of the FAM, VIC/HEX & Internal Control (ROX) channels have a Ct value or Ct ＞ 40;
Positive Control: FAM, VIC/HEX & Internal Control (ROX) channels Ct ≤ 30;
The above two items should be met simultaneously in one experiment. Otherwise, the experiment is invalid and should be repeated.
Data analysis and interpretation:
FAM (UP) | VIC/HEX (UU) | ROX (IC) | Interpretation test results | |
1 | - | - | - | Result invalid,test again |
| 2 | + | - | +/- | UP(+) |
| 3 | - | + | +/- | UU(+) |
| 4 | + | + | +/- | UP and UU(+) |
| 5 | - | - | + | Negative(-) |
NOTE: ‘+’ is positive, ‘-’ is negative.
Operation details please check from IFU!
