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Biokey UP UU Real Time PCR Detection Kit Lyophilized 96 Tests/Kit

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Biokey UP UU Real Time PCR Detection Kit Lyophilized 96 Tests/Kit

City & Province guangzhou
Categories Dyestuff Intermediates
InquireNow

Product Details

UP+UU real-time PCR detection kit (lyophilized)
--96 tests/kit

 
Intended use:
 

This kit, adopting Real-Time PCR-Fluorescence technology, is used for detection of Ureaplasma parvum and Ureaplasma urealyticum(UP+UU). It can be used for the laboratory diagnosis and monitoring of UP+UU infection. The test results are only for clinical reference, not for case confirmation or exclusion.

 

Principle:
 

The specific primers and specific fluorescent probes are designed for the nucleic acid conserved region of UP+UU. The PCR reaction solution and real-time fluorescent quantitative PCR detection technology is applied on the fluorescence quantitative PCR instrument to realize the rapid detection of UP+UU through the change of fluorescence signal. The detection system contains a human internal control (IC) gene, which is used to monitor whether the specimens and the process of nucleic acid extraction is qualified.

 

UP+UU product lyophilization pacakage:

 

 
Main components:

 

No.ComponentsBIK-QL-H0022

1

PCR Enzyme Mix (Lyophilized)

96 tests/bottle

2UP+UU Primer-Probe Mix100 μL/vial
3Enzyme Mix Buffer (5×)400 μL/vial
4Positive Control100 μL/tube
5Negative Control100 μL/tube
6RNase-free water1mL/tube
7Paraffin oil1.5mL/tube*2

  

Note:Do not mix the components from different batches for detection. The Positive Control of UP+UU and internal control were constructed artificially, and they were not infectious.

 

Specimen requirements:
 

1. Specimen type

Male urethral secretions or female cervical secretions.

2. Specimen Preservation

The specimen can be stored for 3 months at -20±5℃ and for a long time below -70℃

 

 

PCR Amplification condition:

 
PCR amplification according to the following procedures:
 

StepCycle numberTemperatureTime
1195℃3min
24095℃10s
60℃30s collect fluorescent

 

Instrument detection channel selection: FAM and HEX/VIC.
 
Data analysis and interpretation:
 

 

FAM

(UP)

VIC/HEX

(UU)

ROX

(IC)

Interpretation test

results

1

-

-

-

Result invalid,test again

2+-+/-UP(+)
3-++/-UU(+)
4+++/-UP and UU(+)
5--+Negative(-)

 

NOTE: ‘+’ is positive, ‘-’ is negative.

 

 

 Operation details please check from IFU!

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