Microprocessor Controller Stainless Steel LAI-3T Anaerobic
Workstation For Lab Cell Culture
Recommendations Before Entering the Transfer Chamber
- Make a list of the things you require to limit the amount of times
you go into and out of the chamber. Too many times wastes gas and
time and leads to oxygen contamination.
- Before bringing your samples or equipment into the anaerobic
chambers, purge them of oxygen by bringing them into the transfer
chamber. Then cycle the transfer chamber between vacuum and gas mix
(95%N2/ 5% H2) no less than three times before you open the inner
chamber. Make sure the chamber isn’t under the vacuum cycle when
you open the door. Remember: Always have the inner door closed and
sealed!
- Bring any required equipment into the chamber at least a night
before you plan to begin an experiment.
- Purge or degas buffers/solutions for 30 minutes to an hour with an
inert gas such as argon or nitrogen before you bring them in to the
intake chamber.
- Sometimes the catalyst ‘stops working’ and the oxygen detector
starts to sense oxygen. Don’t worry, just bake and renew your
catalyst as recommended.
- If you are working with a stable enough protein requiring disulfide
reduction, leave the protein to incubate anaerobically with the
reductant. Then dialyze the reductant away in the chamber
overnight. In place of dialysis, use a desalting spin column to
quickly remove any residual reductant.
- If the protein is not stable enough for overnight dialysis in the
chamber, briefly purge it with an inert gas, and then equilibrate
in the chamber 30 minutes to an hour prior to disulfide reduction.
Model | LAI-3T |
Time for creating anaerobic state in sample chamber | <5 minutes |
Time for creating anaerobic state in operation chamber | <1 hr |
Anaerobic environment maintenance time | >12 hrs.(when no supply of mixed gas) |
Temperature Range | RT+3~60℃ |
Temperature Stability | <±0.3℃ |
Temperature Uniformity | <±1 ℃ |
Display Resolution | 0.1℃ |
Timing Range | 1~9999min |
Power Rating | 600W |
Power Supply | AC 220V,50HZ |
Net/Gross Weight(kg) | 240/320 |
Interior Chamber Size(W×D×H)cm | 30×19×29 |
Operation Chamber Size (W×D×H)cm | 82×66×67 |
Exterior Size (W×D×H)cm | 126×73×138 |
Package Size(W×D×H)cm | 139×92×156 |