Virus PRV GB GE Swine Influenza Antibody Rapid Test Kit 50 Tests/Kit

Porcine Pseudorabies Virus(PRV) gB or gE Antibody Rapid Test Kit 50 Tests/Kit
 

1. Principle

This test strip is based on colloidal gold immuno-chromatography technique to detect porcine pseudorabies virus gB antibody in porcine serum, plasma and whole blood qualitatively. When testing, porcine pseudorabies virus gB antibody in sample combine with antigen coated by colloid gold forming complexes, moving forward to the other head. When reaching the T-line, the specific antigen on the membrane capture the complexes and appear a T line, C line appears, means the test is valid.

2. Specifications: 50 tests/kit

3. Components

4. Sample preparation

4.1 Whole blood
Collect anticoagulated whole blood, use for that very day;
4.2 Serum
Collect blood, put at 37℃for 1-2h, take the supernatant, centrifuge at 1500r/min for 10min,separate the serum. The serum can store for 2-3 days at 4℃, for long term storage, store at -20℃in frozen.

5. Operation procedures

1) Read the instructions carefully and return the test card and sample to room temperature before use.
2) Take out the test card, draw the sample with a dropper, and drop 2-3 drops into the "S" hole; or use a micropipette to transfer 70ul of the sample to the hole marked "S".
3) Read the results in 15 minutes at room temperature.

6. Results

Negative(-): no color reaction on Test Line (T Line),only on Control Line(C Line) being seen red color reaction. It means there is no porcine pseudorabies virus gB antibody in sample or the levels is lower than the detection threshold;
Positive(+): Both on T Line and C line being seen red color reaction. It means there is porcine pseudorabies virus gB antibody in sample;
Invalid: No color reaction on C Line. It means the operation is wrong or the test card is invalid.

7. Notice

1) The test card can only be used once at room temperature, please do not reuse or use expired test cards.
2) Bring all reagents and samples to room temperature before use.
3) Be careful when preparing samples, wear gloves and a mask.
4) Whole blood needs to be added with anticoagulant and cannot be frozen; serum samples can be frozen to avoid repeated freezing and thawing.
5) This product is only for rapid veterinary in vitro diagnosis, and cannot be quantified, but only qualitatively tested.
6) Product test results are for reference only, please refer to relevant national standard methods for confirmation.

Storage and expiry date
Storage: Store at 4-30℃ in dark, sealed, dry place, no frozen.
Expiry date: 12 months; date of production is on box.

Clinical symptoms of porcine pseudorabies virus

The clinical manifestations of pseudorabies virus mainly depend on the virulence and amount of infection of the virus, as well as the age of infected pigs. Among them, the age of infected pigs is the most important. Like herpesviruses of other animals, young pigs are the most ill after being infected with pseudorabies virus.
Newborn piglets infected with pseudorabies virus will cause a large number of deaths. Newborn piglets will behave normally on the first day of clinical diagnosis, and become ill from the second day. The peak period of death is 3 to 5 days, and some whole litters will die. At the same time, the infected piglets showed obvious neurological symptoms, lethargy, whining, vomiting, diarrhea, and died within 1 to 2 days once the disease occurred. At autopsy, the kidneys were mainly covered with needle like bleeding points, sometimes pulmonary edema, meningeal surface congestion and bleeding were seen. Piglets less than 15 days old infected with the disease are very serious, and the morbidity and mortality rate can reach 100%. Piglets suddenly get sick, their body temperature rises to more than 41 ℃, they are extremely tired, shivering, uncoordinated movements, convulsions, vomiting, diarrhea, and rarely recover. Weaned piglets infected with pseudorabies virus have a incidence rate of about 20%~40% and a mortality rate of about 10%~20%. The main manifestations are neurological symptoms, diarrhea, vomiting, etc. Adult pigs are generally recessive infection, and the symptoms are mild and easy to recover. The main symptoms are fever and depression. Some sick pigs vomit and cough, and generally recover completely within 4 to 8 days. Pregnant sows may miscarry, give birth to mummified fetuses or stillbirths, of which stillbirths are mainly seen in first born sows or multiparous sows, and there is no strict seasonal pattern, but they often occur in cold seasons, i.e. late winter and early spring.
Another characteristic of pseudorabies is the sterility of breeding pigs. Sows are sterile after repeated mating, and the rate of return to estrus is as high as 90%. Some sows can not be mated after repeated mating for several times. In addition, boars infected with pseudorabies virus showed swollen and atrophic testes and lost the ability of breeding.

 
Q1: How long will it take for you to ship?
A1: Usually ships within 7 working days.
 
Q2: Do you support OEM/ODM?
A2: can be supported. We can customize according to your specific needs and specific quantities.
 
Q3: How is your factory doing in terms of quality control?
A3: We have ISO9001 certification and ISO13485 certification, so far all the production process, we have standard rules, we comply with the relevant behavior and laws of the government.
 
Q4: Is after-sales service guaranteed?
A4: We provide professional online technical after-sales service. If the product fails during the experiment, we can provide
one-to-one guidance through telephone, video and other forms.
 
Q5: What is your minimum order quantity?
A5: 1Kit.
 
Q6: What is the shipping method?
A6: It can be shipped by express (FEDEX, UPS, DHL, EMS, etc.) or by air and ground.Please confirm with us before placing an order.