Porcine Circovirus Veterinary Elisa Kit Antibody Fast Detection

Porcine Circovirus Veterinary Elisa Kit Antibody Fast Detection

Porcine Circovirus type 2 Antibody Diagnostic ELISA Kit for Pig 192 Wells/Kit

1. Porcine Circovirus type 2 Antibody Diagnostic ELISA Kit Principle
GreenSpring® PCV2 Antibody ELISA Detection Kits are made from antigen-coated microtiter plates (coated with PCV antigen) and other reagents. It applies the principle of solid-phase ELISA to PCV-Ab in serum, and then adds an enzyme conjugate to specifically bind to the complex of coated antigen + PCV-Ab + enzyme-labeled anti-pig IgG antibody on the microplate. With TMB substrate, it produces a certain amount of color. The depth of color is related to the content of PCV-Ab. When the color value is greater than the critical value, the pigs are well inoculated or have natural infection.

2. Porcine Circovirus type 2 Antibody Diagnostic ELISA Kit Components

3. Procedure

1. Remove the coated plate (removable) and record the sample position on the worksheet. Set 2 wells of negative control serum, add undiluted negative control serum, 2 wells of positive control serum, add undiluted positive control serum, 100 μL/well. Others are sample wells, add diluted samples, each 100μL (both single and double wells) -well test is no problem).
2. Mix gently, cover and incubate at 37°C for 30 minutes.
3. Remove the adhesive foil. Pour the liquid out of the wells, fully add the diluted wash buffer to each well, and pour out directly without standing. Repeat 3 times, the last time pat dry on absorbent paper.
4. Add 100 μL of enzyme conjugate to each well.
5. Cover the board with new adhesive foil. Incubate at 37°C for 30 minutes.
6. Repeat step 3 (washing).
7. Add 100ul of substrate to each well, mix well, and incubate at 37°C for 10 minutes in the dark.
8. Add 50 μL of stop solution to each well, mix gently, and measure the result.
9. Measure the OD value of each well with a photometer at dual wavelengths 450nm/630nm.

4. Results

For the assay to be valid, the mean OD value of the positive control wells must be greater than or equal to 0.6, and the mean OD value of the negative control wells must be less than 0.1. Otherwise, the test is invalid and needs to be re-tested.
The result is judged by the S/P value,
S/P=(sample OD450/630- NCx(—))/(PCx(—)- NCx(—)), NCx(—) represents the average OD450/630 value of the negative control, and PCx(—) represents the average OD450 of the positive control /630 value
If S/P ≥ 0.2, it is positive; if it is less than 0.2, it is negative.

5. Product performance

1. Specificity: Use this kit to detect the reference serum, and the compliance rate reaches 100%.
2. Sensitivity: up to 1:5120.
3. Accuracy: CV(%) is not more than 8%.
4. Stability: 12 months at 2℃~8℃ or 3 days at 37℃, the results can meet the above three standards.

6. User Notices and Warnings

1. This kit is for research use only.
2. Do not use expired reagents, and do not mix reagents from different batches.
3. Please read the manual carefully before use.
4. The experimental garbage should be sterilized by high pressure steam at 121℃ for 30 minutes, or treated with 5.0g/L sodium hypochlorite disinfectant for 30 minutes and then discarded.
5. Microplates removed from refrigeration should be equilibrated and allowed to dry at room temperature before opening. Return unused MicroWell plates to dry foil bags and seal at 4 °C. Unused liquid reagents should be covered and stored together with other components at 2-8°C away from light.
6. If the 20X Concentrated Wash Buffer crystallizes, it is a normal phenomenon, put it at 37℃ to dissolve.
7. Micropipettes should be used to add samples and reagents and should be frequently demonstrated for accuracy.
8. When adding washing buffer, it should be topped up but not overflowed to avoid free enzyme or cross-contamination between wells.
9. The termination solution is corrosive, immediately rinse with plenty of water when it comes into contact with skin or clothes.

Specifications: 96 holes × 2.
Validity: 12 months.
Storage: 2~8℃, keep away from light.
Production date: on the outer packaging of the kit.

The incubation period of PCV infection is long. Even if it is infected in the embryonic stage or early postnatal stage, the symptoms will appear gradually after weaning. PVC-2 infection can cause the following diseases:

Multiple system failure syndrome after weaning in pigs

It usually occurs in weaned piglets. It was first reported in 1997, and then reported in America, Europe and Asia. It has been confirmed that PVC-2 is an important pathogen of post-weaning multiple system failure syndrome in pigs. The mixed infection and immune stimulation of reproductive and respiratory syndrome virus, parvovirus, pseudorabies virus and other pathogens can aggravate the harm of the disease.
The affected pigs showed poor spirit, poor appetite, slightly high body temperature, muscle weakness, dysentery, dyspnea, eyelid edema, jaundice, anemia, emaciation, poor growth and development. There was a big difference in weight with the same age pigs, skin eczema, systemic lymph node disease, especially the swelling of inguinal, mesenteric, bronchial and mediastinal lymph nodes. The incidence rate was 5% - 30%, and the mortality rate was 5% - 40%, The convalescent pig becomes a stiff pig.

Dermatitis and nephrotic syndrome

It usually occurs in pigs aged 8-18 weeks. It was first reported in the United Kingdom in 1993, and then in the United States, Europe, South Africa and Asia. The incidence rate is 0.15% - 2%, sometimes as high as 7%. The main symptoms are irregular patches with red and purple bulges on the perineum and limb skin. The affected pigs showed subcutaneous edema, loss of appetite, and sometimes increased body temperature. They usually die within 3 days, sometimes lasting for 2-3 weeks.

Proliferative necrotizing interstitial pneumonia

This disease mainly harms pigs aged 6-14 weeks. The incidence rate is 2% - 30%, and the mortality is 4% - 10%.

Reproductive barrier

It leads to an increase in the oestrus rate of sows, mummification, miscarriage, stillbirth and weak birth.

Q1: When will it be shipped?
A1: We will ship the goods for you as soon as possible within 7 working days after receiving the payment. (In case of external factors such as the epidemic, the delivery may be delayed)

Q2: Does it support OEM/ODM?
A2: It can be supported, but the specific quantity needs to be more than 100,000 pieces, which is convenient for customized products.

Q3: How is your factory doing in terms of quality control?
A3: We have nationally certified ISO9001 and ISO13485. Our production process conforms to standard procedures to ensure optimum product quality.

Q4: How to provide after-sales service?
A4: We provide professional online technical after-sales service. We can provide you with one-on-one guidance via video, telephone, etc.

Q5: What is the payment method?
A5: We receive payment by T/T.

Q6: How to ship?
A6: Choose the best shipping method for you by getting quotes from our many cooperative carriers, and also ship according to your requirements.