Anti-Cotinine Mouse Monoclonal Antibody Purification 3.91mg/mL

Purified Anti-Cotinine Mouse Monoclonal Antibody 3.91mg/mL

Background:

Cotinine is the first-stage metabolite of nicotine, a toxic alkaloid that produces stimulation of the autonomic ganglia and central nervous system when in humans. Nicotine is a drug to which virtually every member of a tobacco-smoking society is exposed whether through direct contact or second-hand inhalation. In addition to tobacco, nicotine is also commercially available as the active ingredient in smoking replacement therapies such as nicotine gum, transdermal patches and nasal sprays. In a 24-hour urine, approximately 5% of a nicotine dose is excreted as unchanged drug with 10% as cotinine and 35% as hydroxycotinine; the concentrations of other metabolites are believed to account for less than 5%.1 While cotinine is thought to be an inactive metabolite, it’s elimination profile is more stable than that of nicotine which is largely urine pH dependent. As a result, cotinine is considered a good biological marker for determining nicotine use. The plasma half-life of nicotine is approximately 60 minutes following inhalation or parenteral administration.2 Nicotine and cotinine are rapidly eliminated by the kidney; the window of detection for cotinine in urine at a cutoff level of 200 ng/mL is expected to be up to 2-3 days after nicotine use. The COT One Step Cotinine Test Device (Urine) is a rapid urine screening test that can be performed without the use of an instrument. The test utilizes a monoclonal antibody to selectively detect elevated levels of Cotinine in urine. The COT One Step Cotinine Test Device (Urine) yields a positive result when the Cotinine in urine exceeds 200 ng/mL.

Specification:

Clonality		Monoclonal
Isotype		IgG1
Host		Host: Mouse Source: Ascites
Specificity		Cotinine
Purity/Purification		DEAE chromatography
Form/Format		Purified, Liquid
Concentration		3.91 mg/ml (lot specific)
Immunogen		Cotinine-BSA
Affinity Constant		Not determined
Buffer		15mM phosphate, pH 7.2 containing 0.85% Sodium chloride
Preservative		NaN3. 0.05% Sodium azide
Warning		This product contains sodium azide, which has been classified as Xn (Harmful), in European Directive 67/548/EEC in the concentration range of 0.1 - 1.0 %. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains.
Preparation and Storage		Store at 2-8 degree C.
ISO Certification		Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes		Small volumes of anti-Cotinine antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.