Beta-lactams, Tetracyclines, Cefalexin and Ceftiofur Combo rapid
test strip
Catalogue No.: GF130AG1
[Brief]
The Beta-lactams, Tetracyclines, Cefalexin and Ceftiofur Combo
rapid test strip, based on Colloidal gold immunochromatography
assay method, is used for testing Beta-lactams, Tetracyclines,
Cefalexin and Ceftiofur residues in dairy products qualitatively.
The whole test only need 10 minutes, easy to operate with high
sensitivity.
[Specifications] 96 pieces per kit
[Components]
Code | Components | Quantity |
1 | Test strip | 8 strips/bottle |
2 | Red dry powder micro-well | 8 pieces/bottle |
3 | Instructions | 1 piece |
[Application]
Dairy products: fresh milk, pasteurized milk, pure cow/sheep milk,
whole milk powder (dissolve 1g of milk powder in 9ml of pure water
and mix thoroughly)
[Detection limit] (See Tablet 1)
[Production date and batch number] Please refer to the inner and outer packaging boxes for details.
[Principle]
The Beta-lactams, Tetracyclines, Cefalexin and Ceftiofur Combo
rapid test strip is based on competitive inhibition
immuno-chromatographic principle. The antibiotics in the sample
bind to colloidal gold labeled specific antibiotic monoclonal
antibodies during the flow process, inhibiting the binding of
antibodies to the antibiotic BSA conjugate on the NC membrane
testing line, resulting in a change in the color of the testing
line.
[Required equipment and consumables]
Instrument: 1 set of Incubator; micropipette
[Operation procedures]
1) Read the manual carefully before testing, return test strips and
sample into room temperature before use.
2) Take bottles needed from the kit package, take out required
microwells and strips, making proper marks, please use it in 1
hour. Seal the cap of the bottles immediately, avoid remaining
reagents getting moisture.
3) Use Micro-pipette to take 200µl of the samples into the microwells, then slowly and repeatedly
aspirate for 5 times until the mixture is even and there is no
solid substance judged by naked eyes (this is a very important
step).
4) Incubate at (40±2℃) for 5min, then insert the strip into
micro-well, put the end with MAX down into the liquid.
5) Insert the strip into the liquid for 5min, read the results
according to the schematic diagram. Please read the results within
1 minute. Interpretation at other times is invalid.
[Test Result Interpretation]
Color depth comparison of T line and C line | Result judgment | Result analysis |
T line > C line | Negative | antibiotics residue is lower than detection limit in sample |
T line = C line | Weak positive | antibiotics residue is equal to detection limit in sample |
T line < C line or T line is invisible | Positive | antibiotics residue is higher than detection limit in sample |
C line is invisible | Invalid | The test strip is invalid, test again |
[Results schematic diagram]
[Precautions]
1) The test strip is disposable at room temperature; Do not use
expired test strips.
2) Please seal the bottle after taking out required test strips and
micro-wells. If can not use all 8 microwells at once, cup the rest
and put it back to bottle and sealed.
3) Do not touch the white membrane surface in the middle of test
strips, avoid sunlight and fan blowing directly.
4) The milk sample must be a uniform liquid without clumping,
souring, or sedimentation.
5) If appear a positive result, recommend use a new strip to test
again.
6) For milk sample with high fat content, chromatography is slow,
recommending extension the second step for another 1 min.
7). The results are for reference only. If confirmation is
required, please refer to relevant national standards and methods;
8). If you encounter any problems during the testing, please
contact the supplier.
[Safety performance and garbage disposal]
The test strips and packaging are non-toxic and harmless. After
use, dispose of them as dry waste
[Storage and expiry date]
Storage: Store at 2-8 ℃ in sealed, dry, and dark place, no frozen.
Expiry date: 12 months
Shenzhen Lvshiyuan Biotechnology Co., Ltd
D Building, National Biological Industrial Park of Marinelife, No.2
Binhai Road, Dapeng, Shenzhen, 518120 China
Tel. 86-755-28438788 Fax 86-755-28938800
Email: info@lsybt.com www.lsybt.com
Tablet 1
Beta-lactams | Detection limit µg/kg(ppb) |
Penicillin G | 2~4 |
Amoxicillin | 2~3 |
Ampicillin | 2~3 |
Oxacillin | 5~7 |
Cloxacillin | 4~6 |
Piperacillin | 6~8 |
Nafcillin | 20~30 |
Cefquinome | 8~15 |
cephalothin | 30~40 |
Cefazolin | 40~50 |
Cephalonium | 8~10 |
Ceftriaxone | 40~50 |
Ceftiofur | 15 |
Cefalexin | 15 |
Tetracyclines | Detection limit µg/kg(ppb) |
Tetracycline | 10-15 |
Oxytetracycline | 10-15 |
Chlortetracycline | 10-15 |
Doxycycline | 20-25 |