Shenzhen Lvshiyuan Biotechnology Co.,Ltd |
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TheTrenbolon ELISA Test Kit
Catalog No. LSY-10068
1. Principle
This test kit is based on the indirect competitive enzyme immunoassay for the detection of theTrenbolon in the sample. The coupling antigen is pre-coated on the micro-well stripes. TheTrenbolon in the sample and the coupling antigen pre-coated on the micro-well stripes compete for the anti-Trenbolon antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with theTrenbolon in it. This value is compared to the standard curve and theTrenbolon concentration is subsequently obtained.
2. Technical specifications
Sensitivity: 0.03ppb
Incubation Temperature: 25℃
Incubation Time: 30min~15min
Detection limit:
Tissue 1.5ppb
Milk,urine 0.6ppb
Milk powder 1.2ppb
Recovery rate
Tissue, milk, urine, milk powder 70%~120%
Cross-reaction rate:
TheTrenbolon 100%
3. Components
1 | Micro-well strips | 12 strips with 8 removable wells each | |
2 | 6× standard solution (1mL each) | 0ppb | 0.03ppb |
0.06ppb | 0.12ppb | ||
0.24ppb | 0.48ppb | ||
3 | Spiking standard solution | 1ml | 100ppb |
4 | Enzyme conjugate | 7ml | red cap |
5 | Antibody working solution | 7ml | blue cap |
6 | Substrate A | 7ml | white cap |
7 | Substrate B | 7ml | black cap |
8 | Stop solution | 7ml | yellow cap |
9 | 10× Sample diluent | 50ml | transparent cap |
10 | 20× concentrated washing buffer | 15ml | white cap |
4. Materials required but not provided
5. Sample pre-treatment
Instructions
The following points must be dealt with before the pre-treatment of any kind of sample:
1) Only the disposable tips can be used for the experiments and the tips must be changed when used for absorbing different reagents;
2) Before the experiment, each experimental equipment must be checked to be clean and should be re-cleaned if necessary, in order to avoid the contamination which interferes with the experimental results.
Solution preparation before sample pre-treatment
1) 50% Methanol solution: 1 part of Methanol + 1 part of deionized water (100ml anhydrous methanol + 100ml deionized water), mix it evenly.
2) 0.01M NaOH solution: Weigh 0.04g solid sodium hydroxide (NaCl) and add deionized water to make the volume to 100ml.
3) Sample diluent: 1 part of 10× Sample diluent + 9 parts of deionized water, mix it evenly.
5.1 Tissue sample preparation
1) Take 1± 0.05 g of the homogenized tissue sample into 10mL/15mL/50mL centrifuge tube;
2) Add 4mL 50% Methanol solution, vortex for 3min; centrifuge at above 4000 r/min at room temperature (20 - 25 ℃) for 5 minutes;
3) Take 100 µl up-layer clear liquid, add 900 µl Sample diluent,mix it evenly;
4) Take 50uL above liquid for analysis.
Fold of dilution of the sample: 40
5.2 Milk and Urine sample preparation
1) Take 1ml of the milk or urine sample into 10mL/15mL/50mL plastic centrifuge tube;
2) Add 4mL 0.01M NaOH solution, vortex for 3min;
3) Centrifuge at above 4000 r/min at room temperature (20 - 25 ℃) for 5 minutes;
4) Take 200 µl up-layer clear liquid, add 600 µl Sample diluent,mix it evenly;
5) Take 50uL above liquid for analysis.
Fold of dilution of the sample: 20
5.3 Milk powder sample preparation
1) Take 1g of the milk powder sample into 10mL/15mL/50mL plastic centrifuge tube;
2) Add 4mL 0.01M NaOH solution, vortex for 3min;
3) Centrifuge at above 4000 r/min at room temperature (20 - 25 ℃) for 5 minutes;
4) Take 100 µl up-layer clear liquid, add 900 µl Sample diluent,mix it evenly;
4) Take 50uL above liquid for analysis.
Fold of dilution of the sample: 40