Using high performance liquid chromatography (HPLC), the stationary
phase used is a weakly acidiccation exchanger. Glycosylation of
hemoglobin will result in the loss of cations on the surface of
hemoglobin without being charged, and non glycosylated hemoglobin
has a positive charge. According to the different charged
properties of hemoglobin, eluents with different pH values and
ionic strengths are used sequentially, and several hemoglobin
components including HbA1c are separated by high performance liquid
chromatography. Each component is detected by a single wavelength
colorimeter, and the detected data is processed by a computer to
calculate the percentage content of HbA1c in the total hemoglobin.
Specification
Instrument | Manufacturer | Lanovation |
Manufacture place | China |
Model | LD-560 |
Dection information | Principle | HPLC |
Detection method | Dual wavelengh colorimetry |
Wavelength | 420nm |
Test time | 90 Seconds |
Test Speed throughtput | 40 samples per hour |
Sample information | Sample types | Whole blood or Pre-diluent sample |
Test blood volume | 5μL in whole blood mode 150μL in pre-diluted mode |
Sample capacity | 20 samples |
Sample loading method | Continuous automatic sample loading |
Mixer | No, but probe mixing samples |
Reagent and other consumble | Column test quantity | 3000 |
Reagent pack | 600T(Reagent)/ 3000T(Column) separate |
Eluent | 2 |
Operation and character information | Operation screen | 10 inch Full color LCD display |
Data storage | 5000 |
Built-in Thermal Printer | Yes |
Performance information | CV | <2% |
Test range | 3.0%~18.0% |
Package Information | Dimensions | 378(L) ×380(W)×510(H)mm |
Weight | 28kg |
Warning
- This instrument is only intended for use by the physicians or
qualified professionals trained by Labnovation or Labnovation’s
agents.
- This instrument can only carry out the instrument inspection
service or any spare parts service provided by Labnovation or
Labnovation’s agents.
- It is important for the hospital or organization that uses this
instrument to execute a reasonable service/maintenance plan.
Neglect of service/maintenance may cause instrument breakdown or
injury to human health.
- If any problem occurs, please perform the troubleshooting
procedures in accordance with Chapter 8. If the problem still
exists, please contact the Labnovation service department for help.
- Please make sure that this analyzer is used under the conditions
specified in the instruction manual. The incorrect instruction may
result in abnormal operation of the analyzer, unreliable measuring
results, and possibly cause damage to parts of the analyzer or even
personal injury.
- The instrument should be inspected strictly before unpacking. In
order to avoid breakage in transportation, the instrument is
carefully packaged. When receiving the analyzer, please carefully
check if there is physical damage in the packaging. If the
packaging is damaged, please contact our services personnel.
Reagents and Consumables
The analyzer is recommended to be used in conjunction with the
Glycosylated Hemoglobin Test Reagent Kit (high performance liquid
chromatography (HPLC method)) produced by our company, which
includes the following components:
Eluent A/Eluent B/Eluent C(Variant Mode) | Adjusts the pH of the solution for the elution of glycosylated
hemoglobin and non glycated hemoglobin. |
Hemolysis Reagent | Used to dissolve red blood cells and release hemoglobin. |
Chromatography Column | Used for separation and elution of glycosylated hemoglobin and non
glycated. |
Hemoglobin Filter | Used to initially filter the sample after hemolysis. |
Paper Rolls | Prints a report of the test results. |