Thermosensitive Alkaline Phosphatase
Cat. No./Spec.: E1013-A/300 U; E1013-B/1,000 U; E1013-C/5,000 U
Concentration: 1 U/µL
Product Description
Thermosensitive Alkaline Phosphatase is a novel alkaline
phosphatase that exhibits activity in commonly used restriction
endonuclease buffers as well as PCR buffers. This enzyme catalyzes
the release of 5' and 3' phosphate groups in DNA, RNA, and
nucleotides. Additionally, it can remove phosphate groups from
proteins. Thermosensitive Alkaline Phosphatase is capable of
dephosphorylating all types of DNA termini within 10 minutes at
37°C. The enzyme is inactivated by heat at 75°C within 5 minutes.
Consequently, there is no need to remove the alkaline phosphatase
before ligation.
Storage Condition & Shelf Life
Store at -20°C. The product is valid for 2 years.
Source
Recombinant E. coli strain containing the alkaline phosphatase gene
cloned from bacteria.
Unit Definition
One unit is defined as the amount of enzyme required to
dephosphorylate the 5'-ends of 1 μg of linearized pUC57 DNA in DSAP
Buffer at 37°C within 10 minutes.
Scope of Application
- Dephosphorylation of cloned vector DNA to prevent
re-circularization during ligation.
- Simultaneous enzymatic cleavage and dephosphorylation of vector
DNA.
- Purification of PCR products: nucleotide degradation of PCR
products before sequencing.
- Depehosphorylation of the 5'-ends of nucleic acids before T4
polynucleotide kinase labeling.
- Other applications requiring dephosphorylation of DNA and RNA
substrates.
- Dephosphorylation of proteins.