T7 RNA Polymerase
Cat. No./Spec.: E1022-A/5,000 U
Concentration: 20 U/µL
Product Description
Phage T7 RNA polymerase is a DNA-dependent RNA polymerase that has
strict specificity for its corresponding double-stranded promoter.
It catalyzes the synthesis of RNA in the 5'→3' direction downstream
of the promoter, starting from the promoter on single-stranded DNA
or double-stranded DNA.
Storage Condition & Shelf Life
Store at -20°C.
Source
Recombinant E. coli strain containing the cloned gene encoding this
enzyme.
Unit Definition
A unit is defined as the amount of enzyme required to incorporate 1
nmol of AMP into a polynucleotide substrate within 60 minutes at
37°C.
Features
- Capable of incorporating modified nucleotides (such as
aminoallyl, biotin, fluorescein, digoxigenin-labeled nucleotides).
Scope of Application
The synthesis of unlabeled and labeled RNA can be used for:
• Hybridization, in vitro RNA translation
• As a substrate in aRNA, siRNA, RNase protection assays, and as a
template for genomic DNA sequencing
• RNA splicing in studies of RNA secondary structure and
RNA-protein interactions.
