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GDSlyo One-step Probe RT-qPCR Kit
Cat. No./Spec.
| Cat. No. | V5013-A | V5013-B | V5013-C |
| 20-μl reaction Nos. | 200 rxns | 1,000 rxns | 5,000 rxns |
Components
| Component | V5013-A | V5013-B | V5013-C |
| 5X GDSlyo One-step Buffer [1] | 800 μl | 4 ml | 20 ml |
| GDSlyo One-step Enzyme Mix [2] | 200 μl | 1 ml | 5 ml |
| 3X Excipientc [3] | 1.2 ml | 6 ml | 30 ml |
| 50X ROX Reference Dye 1 [4] | 80 μl | 400 μl | 1 ml × 2 |
| 50X ROX Reference Dye 2 [4] | 80 μl | 400 μl | 1 ml × 2 |
| RNase-free ddH2O | 1 ml × 3 | 15 ml | 75 ml |
[1] Contains dNTP/dUTP mix, Mg2+.
[2] Contains Reverse Transcriptase, RNase inhibitor, Heat-labile UDG and Taq DNA Polymerase.
[3] Lyophilized excipients, protect the activity of enzymes during the lyophilization process, maintain the morphology and long-term storage stability of lyophilized products.
[4] Used to correct the fluorescence signal error between holes. 50X ROX Reference Dye 1 is used for ABI 7900HT/ 7300 real-time PCR System and StepOne Plus; ABI 7500, 7500 Fast real-time PCR System, Stratagene Mx3000P using 50X ROX Reference Dye 2; Roche, bio-rad Real Time PCR Systems do not have to use ROX.
Storage
This reagent should be kept at -20°C.
Description
The GDSlyo One-step Probe RT-qPCR Kit is a glycerol-free one-step RT-qPCR reagent containing lyophilized excipients for the detection of RNA templates that can be lyophilized with a suitable lyophilization process. The difficulties in the development of lyophilized products include three aspects:1. The removal of liquid reagents affects the lyophilized components, and the stability is poor; 2. It is difficult to freeze-dry and mold, and the yield rate is low; 3. Poor long-term storage stability of lyophilized finished products. Through the optimization of functional proteins, the matching and screening of excipients and lyophilization processes, this product successfully solves the above three problems. Through the screening of enzymes and excipients with high stability, the storage stability of this product is consistent with that of glycerin-containing products; By selecting the appropriate lyophilized excipients and matching the lyophilization process, the yield and stability of the lyophilized products are improved, so that the lyophilized products can be stored at room temperature for more than one year. In addition, this product offers the advantages of conventional glycerol reagents, including good multiplex amplification performance, high specificity, and high sensitivity. The dUTP/UDG anti-contamination system is incorporated into the reagents, which can be used at room temperature to eliminate the influence of amplification product contamination on qPCR results and ensure the accuracy of results.
Application
• Used in the production of lyophilized reagents
• Probe gene expression analysis
• Probe Low-copy gene detection
• Probe microarray validation
• Probe gene knockdown validation
Features
• Glycerol free
• One-step RT-qPCR
• This kit is suitable for fluorescence quantification by probe method
• This kit is compatible with many real-time systems
• Hot-start technology brings high specificity and reproducible amplification
• dUTP/UDG system, effectively prevent PCR product contamination
Product Selection Guide
| Cat. No. | V5001/V5002 | V5005/V5006 | V5005L/V5006L | V5007/V5008 | V5007L/V5008L | V5009/V5010 | V5011 |
| Product Name | One-step Probe RT-qPCR Kit | DSPathTM4X One-Step Multiplex Master Mix | DSPathTM4X One-Step Multiplex Master Mix (Lyophilized) | 2X One Step Prime RT-qPCR Mix | 2X One Step Prime RT-qPCR Mix (Lyophilized) | One-step Probe RT-qPCR Kit V2 | One-step Probe RT-qPCR Kit V3 |
| Mixture Format | Enzyme Mix+2X Buffer Mix | 4X All-in Mix | 4X All-in Lyophilized Powder | 2X All-in Mix | 2X All-in Lyophilized Powder | Enzyme Mix+2X Buffer Mix | Enzyme Mix+5X Buffer Mix |
| Mode/Time | Standard/~120 min | Fast/~60 min or Standard/~120 min | Fast/~60 min or Standard/~120 min | Fast/~60 min or Standard/~120 min | Fast/~60 min or Standard/~120 min | Fast/~60 min or Standard/~88 min | Fast/~45min or Standard/~80min |
| RT Temperature | 50~55°C | 48~55°C | 48~55°C | 48~55°C | 48~55°C | 50~55°C | 50~55°C |
| PCR Enzyme | Antibody-modified hotstart Taq | Antibody-modified hotstart Taq | Antibody-modified hotstart Taq | Antibody-modified hotstart Taq | Antibody-modified hotstart Taq | Antibody-modified hotstart Taq | Antibody-modified hotstart Taq |
| Heat-labile UDG | + | + | + | + | + | + | + |
| Sensitivity | Very high | Very high | Very high | Very high | Very high | Ultra high | Ultra high |
Dongsheng Biotech Co., Ltd takes PCR technology as its core, and its products focus on common PCR, qPCR, nucleic acid detection and other fields. Adhering to the quality policy of "unremitting pursuit of continuous innovation, leading technology and customer satisfaction", we provide our customers with cost-effective molecular biology products.
