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Cat. No./Spec.: K010-A/100U; K010-B/200U; K010-C/1,000U
Concentration: 5U/µL
Product Description
Klenow Fragment (3´→ 5´ exo-) is an N-terminal truncation of DNA Polymerase I which retains polymerase activity, but has lost the 5´→ 3´ exonuclease activity and has mutations which abolish the 3´→ 5´ exonuclease activity.
Components
| Component | K010-A (100U) | K010-B (200U) | K010-C (1,000U) |
| Klenow Fragment(3´→5´ exo-)(5U/µL) | 20 µL | 40 µL | 200 µL |
| 10X Klenow Buffer | 1.25 mL | 1.25 mL | 1.25 mL |
Storage Condition & Shelf Life
All reagents should be stored at -20°C. The product is valid for 24 months.
Source
Recombinant E. coli strain.
Unit Definition
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C.
Scope of Application
1. Generates probes using random primers
2. Random priming labeling
3. dA-tailing at 3' end
4. Second strand cDNA synthesis
