High Sensitive Porcine Immunoglobulin M ELISA Kit for Accurate Quantitative Detection

Porcine Immunoglobulin M ELISA Kit with High Spesificity and Sensitivity 

Cat.No E0229Po

Standard Curve Range: 0.05mg/ml - 20mg/ml

Sensitivity: 0.022mg/ml

Size: 96 wells

Storage: Store the reagents at 2-8°C. For over 6-month storage refer to the expiration date keep it at -20°C. Avoid repeated thaw cycles. If individual reagents are opened it is recommended that the kit be used within 1 month.

* This product is for research use only, not for use in diagnosis procedures. It’s highly recommend to read this instruction entirely before use.

Intended Use

This sandwich kit is for the accurate quantitative detection of Porcine Immunoglobulin M (also known as IgM) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Assay Principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Porcine IgM antibody. IgM present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Porcine IgM Antibody is added and binds to IgM in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated IgM antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Porcine IgM. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.