Principle
Soybean Agglutinin ELISA Test Kit is a competitive enzyme
immunoassay for the quantitative analysis of Agglutinin in soybean,
feed, soybean meal, fermented soybean meal, extruded soybean, soy
protein isolate, soy protein concentrated samples.
Soybean Agglutinin ELISA Test Kit incorporates a fast, low-cost
sample prep protocol with high recovery rates.
An antibody with a affinity for Soybean Agglutinin is coated onto
microwells. Standard or sample is added to the appropiate well and
if Soybean Agglutinin is present it will bind to the coated
antibody. Subsequently, Soybean Agglutinin bound to horse-radish
peroxidase (HRP) is added and binds to the antibody not already
occupied by Soybean Agglutinin present in the sample or standard.
After this incubation period, the contents of the wells are
decanted, washed and an HRP substrate is added which develops a
blue color in the present of an enzyme. The resulting color
intensity, has an inverse relationship with the target
concentration in the sample. The levels of Soybean Agglutinin in
the sample are determined by comparison of the color intensity of
unknown samples with the standard curve, which is made using the
Soybean Agglutinin standards provided in the kit.
Technical specifications
Detect Range : 0.20-12mg/g
Specification: 96tests /kit
Reaction temperature: 37°C
Detect time: 30min
For more information, please visit website:
Contact: John
E-mail: info@ecalbio.com
Website: http://www.ecalbio.com/
