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Laboratory Or Hospital High Accuracy CMV IgG Elisa Test Kit

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Laboratory Or Hospital High Accuracy CMV IgG Elisa Test Kit

Country/Region	china
City & Province	beijing beijing
Categories	Orthopedic Supplies

Product Details

CMV IgG Elisa Test Kit

INTENDED USE

CMV IgG Elisa test is intended for the detection and quantitative determination of IgG antibody to CMV in human sera.

Product details	Description
Delivery	Within 48 hours
Packaging Specifications	8 x 12 strips, 96 wells
Country Of Origin	China
Manufacturer	18 months
Preservation method	2℃-8℃
Specimen	Whole blood
Assification	class1
Type	Elisa Test Kit

ASSAY PROCEDURE

Prepare Reagents: Dilute 1 volume of Washing Buffer (20×)with 19 volumes of distilled water, mix well.
Add Samples: Open the foil pouch and remove the Microplate. Set up 1 well as Blank, 2 wells as negative control, 2 wells as positive control. After dispensing 100μL of Sample Diluent , dispense 10μL of sample, 100μL negative control or positive control to the respective wells(without Sample Diluent). Gently vibrating the plate.
Incubate: Cover the Microplate with plate cover and incubate the Microplate in a thermostat-controlled water-bath or microplate incubator at 37℃ for 30 minutes.
Wash the Plate: Remove the plate cover. Aspirate the contents of all wells. Fill the wells with the diluted washing buffer ( 10~20 seconds to soak) then aspirate again. Repeat the procedure for 5 times. Make sure that the rest volume is minimal, by tapping plate onto absorbent paper.
Add Conjugate: Add 100μL of conjugate to each well (except the blank well).
Incubate: Cover the Microplate and incubate the plate at 37℃ for 30 minutes.
Wash the Plate: Repeat the wash procedure as in step 4.
Add Substrate: Add 50μL of Substrate Solution A and 50μL of Substrate Solution B to each well, mix well. Cover and incubate at 37℃ for 15 minutes.
Stop reaction: Add 50μL Stop Solution to each well, mix well.
Read the absorbance at 450 nm. If a dual wavelength measurement is used, the reference wavelength should be selected from 620nm to 690nm.

Storage

• Store at 2-8℃.

• Place unused wells in the zip-lock aluminum foiled pouch and return to 2-8 °C, under which conditions the wells will remain stable for 2 months, or until the labeled expiry date whichever is earlier.

• Seal and return unused calibrators to 2-8 °C, under which conditions the stability will be retained for 1 month, for longer use, store opened calibrators in aliquots and freeze at-20 °C. Avoid multiple freeze-thaw cycles.

• Seal and return all the other unused reagents to 2-8 °C, under which conditions the stability will be retained for 2 months, or until the labeled expiry date, whichever is earlier.