High accuracy Toxo IgM ELISA TEST KIT

INTENDED USE

Toxo IgM Elisa test is intended for the qualitative detection of IgM antibody to TOXO in human serum for the presumptive diagnosis of acute, recent, or reactive TOXO infection.

ASSAY PROCEDURE

• Prepare Reagents: Dilute 1 volume of Washing Buffer (20×)with 19 volumes of distilled water, mix well.
• Add Samples: Open the foil pouch and remove the Microplate. Set up 1 well as Blank, 2 wells as negative control, 2 wells as positive control. After dispensing 100μL of Sample Diluent , dispense 10μL of sample, 100μL negative control or positive control to the respective wells(without Sample Diluent). Gently vibrating the plate.
• Incubate: Cover the Microplate with plate cover and incubate the Microplate in a thermostat-controlled water-bath or microplate incubator at 37℃ for 30 minutes.
• Wash the Plate: Remove the plate cover. Aspirate the contents of all wells. Fill the wells with the diluted washing buffer ( 10~20 seconds to soak) then aspirate again. Repeat the procedure for 5 times. Make sure that the rest volume is minimal, by tapping plate onto absorbent paper.
• Add Conjugate: Add 100μL of conjugate to each well (except the blank well).
• Incubate: Cover the Microplate and incubate the plate at 37℃ for 30 minutes.
• Wash the Plate: Repeat the wash procedure as in step 4.
• Add Substrate: Add 50μL of Substrate Solution A and 50μL of Substrate Solution B to each well, mix well. Cover and incubate at 37℃ for 15 minutes.
• Stop reaction: Add 50μL Stop Solution to each well, mix well.
• Read the absorbance at 450 nm. If a dual wavelength measurement is used, the reference wavelength should be selected from 620nm to 690nm.