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Nucleic Acid Mycoplasma Detection Kit MFV Real Time Quantitative PCR

Guangzhou BioKey Healthy Technology Co.Ltd

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Nucleic Acid Mycoplasma Detection Kit MFV Real Time Quantitative PCR

City & Province guangzhou
Categories Dyestuff Intermediates
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Product Details

Mycoplasma Feline virus (MFV) Nucleic Acid PCR kits Real Time fluorescence quantitative PCR probe kit

 

Intend for Use

This kit is applied  for the specified detection of feline Mycoplasma  in mouth and nasal secretions of Felines/cats.

 

Principle

This kit choose Felines/Cats' Mycoplasma conservative design specific gene probe, the probe can with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, thereby quenching groups out of the 3' end, glow, The nucleic acid of feline Mycoplasma was detected by fluorescence PCR in a completely closed reaction system. 

 

ComponentSpecification
PCR reaction liquid8 tubes
Positive control1 tube
Negative control1 tube

 

 

Storage: keep away from direct light  uner -20±5℃

 

 

 

 

The results of Analysis 

 

 
ResultsCt Value
PositiveCt≤40
NegativeNo Ct value or Ct value=0
Gray area40<Ct≤45

 

Operation details please check from IFU!

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