HPV Aquaculture Test Kit Hepatopancreas Parvovirus Nucleic Acid Test Kit

Hepatopancreas Parvovirus ( HPV ) Nucleic Acid Magnetic Beads Fluorescent Probe PCR test kit

 
Intended use:

This kit is suitable for auxiliary diagnosis of hepatopancreas parvovirus (HPV) infection.

Principle:

This kit hepatopancreas parvovirus conservative design specific gene probe, the probe can with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, thereby quenching groups out of the 3' end, glow, The nucleic acid of hepatopancreas parvovirus was detected by fluorescence PCR apparatus in a completely closed reaction system.

Main components:
 

 
Sample requirements:
 

1. Sample type

Edible prawn, machijiming prawn, vannamei and other susceptible prawn tissues

2. Sample collection

About 0.1g tissue samples were taken from prawn samples according to different sizes or infection stages. Among them, whole individuals were taken from larvae and larvae, head and chest of eyes were removed from juveniles under 3CM, gill area was taken from larger juveniles, and gill filaments or heart or lymphatic organs were taken from adult shrimp.

3. Storage and transportation

Samples are transported in ice bags of 2℃~8℃, and repeated freezing and thawing are prohibited. Short-term storage at -20℃, -70℃ can be long-term storage.

PCR amplification and fluorescence detection (PCR detection area):

Put each reaction tube into the PCR instrument and perform PCR amplification according to the following procedures:

FAM is selected for detection channels.

The results of interpretation:

Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.

 Operation details please check from IFU!