DNA Taq Aquaculture Test Kit Grass Carp Reovirus Virus GCRVI Type I PCR Test Kit

DNA Taq polymeraset detect Grass Carp Reovirus virus ( GCRVI ) Type I PCR test kit

 
Intended use:

This kit is suitable for auxiliary diagnosis of Grass Carp Reovirus Type I virus (GCRVI) infection

Principle:
 
This kit uses RNA as a template and primers as a starting point to synthesize cDNA strands complementary to RNA template under the action of high-efficiency reverse transcriptase. Choose peach virus gene conservative design specific probe, the probe can with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, thereby quenching groups out of the 3' end, glow, The nucleic acid of taora virus was detected by fluorescence PCR in a completely closed reaction system.​
 
Main components:
 

1. Sample type

Tissue samples of grass carp and black carp

2. Sample collection

Fry with body length ≤4cm should be taken as a whole fish, if there is yolk sac, it should be removed; All viscera including kidney and brain were taken from 4~6cm fry. Liver, brain, spleen and kidney were taken from fish with body length ≥6cm. 5 fish can be combined into a small sample.

3. Storage and transportation

Samples are transported in ice bags of 2℃~8℃, and repeated freezing and thawing are prohibited. Short-term storage at -20℃, -70℃ can be long-term storage.

PCR amplification and fluorescence detection (PCR detection area):

Put each reaction tube into the PCR instrument and perform PCR amplification according to the following procedures:

FAM is selected for detection channels.
 
The results of interpretation:
 

Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.

 
Product performance index:
 

1. Accuracy

The positive reference materials of testing enterprises were all positive.

2. Minimum detection limit: the detection limit of this kit is 3copies/μL.

3. Precision: Coefficient of variation (CV,%) of intra-batch precision Ct value is ≤5%.

  Operation details please check from IFU!