Avian Influenza Virus ELISA Anti Body Test Kit Diagnostic 96Wells/Kit ISO9001

Avian influenza Virus Antibody Diagnostic ELISA Kit 96Wells/Kit ISO9001

1. Introduction

Avian influenza, also known as true bird flu or European bird flu, is an acute and highly lethal infectious disease caused by type A avian influenza virus (AIV), which is characterized by asymptomatic poisoning and acute infectious death.

This ELISA kit is used to detect avian influenza virus type A antibodies in the serum, plasma or egg yolk of chickens, ducks, turkeys, quails, guinea fowls, geese and other poultry, and can be used for immune effect evaluation and auxiliary diagnosis. .

2. Principle

This kit is composed of AIV antigen pre-coated microplates, antibody working solution, enzyme conjugates, etc. It adopts competitive ELISA (blocking ELISA). When running the test, add diluted sample and antibody working solution to the pre-coated microplate wells. If AIV antibody is present in the sample, it will prevent the antibody working solution from binding to the pre-coated AIV antigen on the microplate. As a result, there is no color reaction in the following steps, otherwise there will be a color reaction. The color depth of the sample is negatively correlated with the specific antibody content in the sample. After the stop solution was added, the color changed to yellow. The absorbance value of each reaction well was measured at 450 nm with a microplate reader. We can then know if avian influenza virus (AIV) type A antibodies are present in the sample.

3. Components

4. Materials required but not provided

1) microplate reader (including 450/630 nm wavelengths).

2) Adjustable Micropipettes

3) 37℃ constant temperature equipment

5. Sample Preparation

1) Bring the 20x Concentrated Wash Buffer back to room temperature (about 25°C) before use, shake to dissolve the precipitated salts, and then dilute 20x with distilled or deionized water.

2) Serum/plasma: Take whole blood of animals, and take serum or plasma according to conventional methods. Serum/plasma should be clarified, without hemolysis and pollution. Samples can be stored at 2~8°C for short-term storage (within 1 week) and -20°C for long-term storage.

Egg yolk: Take 2ml of fresh egg yolk, add 2ml of physiological saline, shake well, centrifuge at 3000r/min for 15min, and take the supernatant as a sample.

3) Dilute serum, plasma or prepared egg yolk samples 5-fold with diluted wash buffer (eg add 10ul of sample to 40ul of diluted wash buffer and mix well). Positive and negative controls do not require dilution.

6. Test procedure

1) Return the kit to room temperature for 30 minutes before use.

2) Take the required number of microplate wells, set 2 negative control wells and 2 positive control wells, seal unused wells, and store at 2~8°C as soon as possible.

3) Negative control is added to negative control, 50ul/well, positive control is added to positive control, 50ul/well; sample well is added to diluted sample 50ul/well;

4) Add antibody working solution to each well, 50ul/well. Mix well and incubate at 37°C for 30 minutes.

5) Pour off the liquid in the wells, fill all wells with wash solution, incubate for 30 seconds and discard. Repeat the wash procedure 5 times as above and pat dry.

6) Add enzyme conjugate to each well, 100ul/well. Incubate at 37°C for 30 minutes.

7) Wash step 5).

8) Add Substrate A: 50ul/well to each well, then add Substrate B: 50ul/well, mix well, and incubate at 37°C for 15 minutes in the dark.

9) Add stop solution: 50ul/well per well, mix well, and use ELISA Reader to measure the A value of each well at 450nm (630nm is the reference).

7. Results

1) Under normal circumstances, the A value of the negative control well is greater than or equal to 1.0;

2) Under normal circumstances, the A value of the positive control well ≤ the A value of the negative control well × 50%;

3) PI (blocking rate) = (1-sample value/negative control average) X 100%,PI ≥ 50%: positive; PI < 50%: negative

4) Non-immunized flocks: Positive results may have infection, which needs to be analyzed in combination with clinical data.

5) Immune flocks: Monitor and record the antibody level of the samples at this time, and analyze the distribution of antibody levels and changes in the immune status of the flocks according to the results.

8. Restrictions

This kit can only qualitatively detect AIV antibodies. The strong, medium and weak antibody levels were roughly evaluated according to the PI value.

9. Notes

1) Wear gloves and work clothes during operation, and strictly implement the disinfection and isolation system. Various experimental wastes should be treated as pollutants

2) The stop solution is corrosive. Avoid contact with skin and clothes. If contact occurs, rinse with tap water.

3) Microplates removed from refrigeration should be equilibrated to dryness at room temperature, and unused microplates should be sealed with desiccant.

4) The washing liquid is easy to crystallize at low temperature, and it will dissolve at room temperature when used.

5) Add wash buffer sufficiently to each well to prevent well-free enzymes from failing to wash

6) Test samples should be fresh.

7) Determination of test results must be based on an ELISA reader.

8) Do not mix reagents from different batches.

Specifications: 5X96, 2X96, 1X96 holes/kit.

Validity: 12 months.

Storage: 2-8℃, keep away from light.

Store microplates at 2-8°C, away from moisture, for 2 months after opening the package.

Q1: How long will it take for you to ship?
A1: Usually ships within 7 working days.

Q2: Do you support OEM/ODM?
A2: can be supported. We can customize according to your specific needs and specific quantities.

Q3: How is your factory doing in terms of quality control?
A3: We have ISO9001 certification and ISO13485 certification, so far all the production process, we have standard rules, we comply with the relevant behavior and laws of the government.

Q4: Is after-sales service guaranteed?
A4: We provide professional online technical after-sales service. If the product fails during the experiment, we can provide
one-to-one guidance through telephone, video and other forms.

Q5: What is your minimum order quantity?
A5: 1Kit.

Q6: What is the shipping method?
A6: It can be shipped by express (FEDEX, UPS, DHL, EMS, etc.) or by air and ground.Please confirm with us before placing an order.