Elisa CE Neutralizing Antibodies Detection Kit For Human Serum / Plasma Sample

KISSH SARS-CoV-2 Neutralizing Antibodies Detection Kit (ELISA)

Test Principle

The SARS-COV-2 Neutralizing Antibody Detection Kit (ELISA) adopts the enzyme-linked immune method to simulate the virus neutralization process to qualitative analyze the new coronavirus neutralizing antibody in human blood samples. The kit contains two key components: HRP labeled recombinant SARS-COV-2 RBD protein (HRP-RBD) and human ACE-2 receptor protein (ACLi-2). Firstly, after the sample and quality control sample were pre incubated with hrp-rbd, the neutralizing antibody of the sample was combined with HRP-RBD to form a mixture of neutralizing antibodies.Then the mixture was added to the enzyme plate pre coated with ACE-2 protein and incubated for a period of time. The unbound 1IRP-R13D and the bound with non neutralizing antibody will bind to ACE-2 protein on the plate, while the mixture of neutralizing antibody will be removed during washing. After the washing step, TMB solution is added. After a period of reaction, the color turns blue. After the termination solution is added, the color turns yellow and the value is read and calculated. The higher the content of SARS-CoV-2 neutralizing antibody in the sample, the lighter the color; the lower the content, the darker the color.

Intended Use