Active Member
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[China]
Address: Office Address: Building 4, No. 51 Jingbei 3rd Road, Jingkai Zone, Zhengzhou City. Henan Province,China.
Contact name:chen
Henan Lantian Medical Supplies Co.,Ltd. |
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The kit is used for qualitative detection of novel coronal virus
neutralizing antibodies in human serum, plasma and whole blood
samples.
Coronal beer is the new coronal virus, the new coronal virus for a
long time. Corona virus. May cause viral pneumonia, the main
clinical symptoms are fever, tiredness, dry cough. There are also
patients with nasal congestion, runny nose, sore throat and
diarrhea. Severe illness usually follows one week with dyspnea
and/or hypoxemia, acute dyspnea syndrome, septic shock, refractory
metabolic adoxis, and coagulation dysfunction.
A new coronavirus infection or vaccination in 2019 will cause an
immune response and antibody production in the blood. Antibodies
are secreted to prevent viral infection. After infection, they
remain in the human circulatory system for months to years, where
they bind quickly and firmly to the pathogen and prevent the virus
from replicating. These antibodies are called neutralizing
antibodies. Neutralization antibody test can determine whether a
person has the ability to prevent viral infection.
The kit uses immune colorimetry. It consists of inspection card
quality management line C, inspection line T and reference line R.
The samples (serum/plasma and whole blood) were determined by
diffusion loading and binding, and the s-RBD manufacturing plate
was combined with the NC immobilized ACE2 to form membrane protein
detection and signal localization lines. When neutralizing
antibodies are present in the sample, they bind to the labeled
S-RBD antigen when passing through the labeled S-RBD antigen.
Neutralizing antibodies prevent S-RBD from binding to ACE2. As a
result, the amount of neutralizing antibodies and the negative
correlation signal value are reduced. At very high concentrations
of neutralizing antibodies, the T-line becomes colorless. Whether
reference lines R and QCC are colored for T lines. Use quality
control line C
If the C line is not colored, the test is invalid and the sample
needs to be tested again.
The kit consists of test cards and sample buffers.
Test card: aluminum foil bag, desiccant, test strip, plastic card
composition. The test strip consists of absorbent paper,
nitrocellulose mixture, sample pad, adhesive pad and rubber plate.
T line (test line) of nitrifying membrane covers ACE2 protein, C
line (QC line) covers Ab of QC line, and R line (reference line)
covers reference Ab. The pad contains the label 2019nCoVAb.
Sample buffer: phosphoric acid, sodium nitrite, etc.
1. Collect serum, plasma or whole blood samples.
2. Sediment and plankton in the sample will affect the experimental
results, so it needs to be removed by centrifugation.
3. Anti-coagulation therapy: loofah anti-coagulation therapy, EDA
anti-coagulation therapy, sodium citrate anti-coagulation therapy
has no obvious effect.
4. Blood collection by experts is needed. Serum/serum/plasma tests
are recommended. In emergency or special circumstances, a patient's
whole blood sample can also be used for rapid testing.
5. Serum and plasma samples should not be stored for more than 8
hours at room temperature.
It can be stored for 5 days at 2-8 ℃ and 6 months below -20℃, but
please avoid repeated freezing and melting. Whole blood samples do
not freeze and need to be stored at 2℃ to 8℃ for 48 hours.
Please read the instructions carefully before testing. Before the
test, all reagents should be returned to room temperature and tests
must be carried out at room temperature.
1. Take the test card out of the box and use it within 1 hour.
2. Add 20L sample (serum, plasma or whole blood) and 2 drops (~
60L) of sample buffer storage into the filling hole of the test
card, and start timing.
3. After 10 to 15 minutes of reaction at room temperature, read the
results. Invalid after 20 minutes.
Test card description:
1. Invalid result: Quality management line (Line C) is invalid and
needs to be tested again.
2. Negative result: The appearance color of test line (T line) is
above the reference line (R line), quality management line (C line)
and reference line (R line).
3. Positive results: the test line (T line) or the red watchband is
not displayed on the red watchband, the test line (T line) is lower
than the reference line (R line), quality management line (C) and
the reference line (R line) with color.
This kit is for qualitative analysis and INVitro diagnostics only.
2. Please add appropriate test samples. With too many or too few
samples, the results may be incorrect.
3. Hemolysis, lipids in the blood, jaundice and sample
contamination may affect the test results, so please try to avoid
them.
4. The test results of the reagent are for clinical reference only,
and should not be used as the sole basis for clinical diagnosis and
treatment. The final diagnosis of the disease is made by combining
all clinical and examination results.
1. This product is only used for Invitro diagnosis.
This product is disposable and can't be reused.
3. Please read the instructions carefully before operation, and
strictly follow the instructions of reagent for experimental
operation.
4. Please avoid conducting the experiment under harsh environmental
conditions (84 disinfectants, sodium hypochlorite, acid, alkali,
acetaldehyde, other corrosive gases with high concentration, dust,
etc.). Laboratory disinfection needs to be carried out after the
experiment.
5. All samples and reagents used shall be considered as potentially
infectious substances and shall be disposed of in accordance with
local regulations.
6. The reagent must be used within the expiry date stated on the
package. To prevent moisture, the test card needs to be removed
from the aluminum foil bag as soon as possible after removal.
