pcr laboratory Cell Insert
Cell Insert
Multiple well plate or dish is made from PS
Permeable supports, also known as cell culture inserts, are an
essential tool for the study of both anchorage-dependent and
independent cell lines.
This product produce a cell culture environment that closely
resembles an in vivo state. Allow polarized cells to carry out
metabolic activities in a more natural manner because the cells
feed both apically and basolaterally.
Application: co-culture, epithelial cell polarity, migration,
invasion, transport and permeability studies.
·Innovative hanging design facilitates pipetting.
·Supplied in multiple well plates: 6-well, 12-well, 24well.
·PC membrane: low adsorption rate, reducing the loss of small
molecular proteins and other compounds
·Transparent PET inserts permit sufficient optical transparency for
visualization of cell outlines by phase contrast microscopy.
· Transparent PET membrane is easy to observe.
·The PC membrane is not easy to be torn or curled, and it is easy
to operate when taking out the chamber.
·Compatible with most solvents used for fixing and dyeing.
Attention:
• Attachment of cells grown on a permeable support (membrane) is
sensitive to the initial seeding density, so the initial use should
be inoculated with different density guarantee optimal growth;
•During the cultivation process, the liquid should be taken through
the gap between the upper layer and the hole. When adding liquid,
be careful and slow to avoid the damage of the membrane;
•Incubation of the permeable support in the medium prior to
culturing the cells can improve cell attachment and distribution.
Guidelines for us:
• Pre-equilibration: After added culture solution to the pores of
the multi-well plate and inserted the insert, please placed it in
the incubator for at least one hour or overnight. • Fix and stain
cells directly in the nest, and use a scalpel to cut the membrane
for long-term storage. Before using please add culture solution to
the pores of the multi-well plate, then insert the insert and add
the culture solution which included cells to the insert.• Before
using please add culture solution to the pores of the multi-well
plate, then insert the insert and add the culture solution which
included cells to the insert.
• Pre-equilibration: After added culture solution to the pores of
the multi-well plate and inserted the insert, please placed it in
the incubator for at least one hour or overnight.
• Check the volume of the culture medium regularly and add fresh
medium as needed.
• The cell layer can be directly fixed and stained in the nest
using basic cytological methods. Note Avoid using solvents that
dissolve the PC film.
• Fix and stain cells directly in the nest, and use a scalpel to
cut the membrane for long-term storage.
Before using please add culture solution to the pores of the
multi-well plate, then insert the insert and add the culture
solution which included cells to the insert.
packing specification | Inserts/plate | Insert Diameter(mm) | Well Volume(ml) | Insert Volume(ml) | Insert Membrane Growth Area(cm²) |
6 | 6 inserts/6well | 24 | 2.6 | 1.5 | 4.67 |
12 | 12 inserts/6well | 12 | 1.5 | 0.5 | 1.12 |
24 | 12 inserts/6well | 6.5 | 0.6 | 0.1 | 0.33 |
100mm dish | 1 inserts/6well | 75 | 13 | 9 | 44 |
Pore Size(um) | Membrane Density(well/cm²) |
0.4 | 1x108 |
3.0 | 2x106 |
5.0 | 4x105 |
8.0 | 1x105 |
50% Transparent PC Membrane Cell Insert, Tissue Culture
Treated(Individually packaged in peelable Tyvek film and
Blister,10/case)
Cat.No. | Type(Well) | Pore Size(um) |
723001 | 6 | 3.0 |
724001 | 12 | 3.0 |
725001 | 24 | 3.0 |
723101 | 6 | 0.4 |
724101 | 12 | 0.4 |
725101 | 24 | 0.4 |
724201 | 12 | 5.0 |
725201 | 24 | 5.0 |
723301 | 6 | 8.0 |
724301 | 12 | 8.0 |
725301 | 24 | 8.0 |
726001 | 100mm dish | 3.0 |
50% Transparent PC Membrane Cell Insert, Non-treated(Individually
packaged in peelable Tyvek film and Blister,10/case)
Cat.No. | Type(Well) | Pore Size(um) |
723011 | 6 | 3.0 |
724011 | 12 | 3.0 |
725011 | 24 | 3.0 |
723111 | 6 | 0.4 |
724111 | 12 | 0.4 |
725111 | 24 | 0.4 |
724211 | 12 | 5.0 |
725211 | 24 | 5.0 |
723311 | 6 | 8.0 |
724311 | 12 | 8.0 |
725311 | 24 | 8.0 |
Transparent PET Membrane Cell Insert, Tissue Culture
Treated(Individually packaged in peelable Tyvek film and
Blister,10/case)
Cat.No. | Type(Well) | Pore Size(um) |
723121 | 6 | 0.4 |
724121 | 12 | 0.4 |
725121 | 24 | 0.4 |
723021 | 6 | 3.0 |
724021 | 12 | 3.0 |
725021 | 24 | 3.0 |
Transparent PET Membrane Cell Insert, Non-treated(Individually
packaged in peelable Tyvek film and Blister,10/case)
Cat.No. | Type(Well) | Pore Size(um) |
723131 | 6 | 0.4 |
724131 | 12 | 0.4 |
725131 | 24 | 0.4 |
723031 | 6 | 3.0 |
724031 | 12 | 3.0 |
725031 | 24 | 3.0 |