Huachenyang (Shenzhen) Technology Co., Ltd |
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ODM PCR Nucleic Acid Purification Kits Magnetic Bead Method
Magnetic bead DNA extraction reagent Medical nucleic acid extraction kit Magnetic bead method
product description:
The HUACHENYANG DNA extraction kit provides a fast and efficient
magnetic bead method to purify and extract DNA (including genomic,
mitochondrial and viral DNA) from preserved tissues, saliva, body
fluids, as well as from oral cavity, cervix, skin cells, bacterial
cells, etc. Biological samples can be stored at room temperature
for up to 30 days in our proprietary storage buffer before
processing without significant loss of DNA yield or quality (more
than 30 days if stored frozen) No phenol/ Chloroform extraction or
alcohol precipitation yields high-quality genomic DNA within 15
minutes, with an average DNA yield of 8 μg per buccal swab.
Purified DNA, approximately 20-30 kb, suitable for downstream
applications such as PCR or other enzymatic reactions
Description:
The main components are magnetic beads, proteinase K, sodium chloride. Magnesium Chloride. Potassium chloride, Tris, etc.
Features:
Using silicon magnetic beads and buffer system with unique
separation function, it can quickly, highly sensitively and
efficiently extract high-purity nucleic acid from samples, and the
extracted and purified nucleic acid can be used for PCR, RT-PCR,
digestion, reverse transcription, and Southern Various common
downstream experiments such as blot, applicable specimen types:
whole blood, tissue homogenate, swab, serum, plasma,
bronchoalveolar lavage fluid, etc. Applicable extraction
instruments: most of the automatic nucleic acid extraction
instruments on the market
benefit:
1. Magnetic bead-based technology isolates genomic DNA without the
need for hazardous chemicals, centrifugation or vacuum manifolds,
phenol and ethanol precipitation.
2. Fast and efficient purification of genomic DNA from human buccal
swabs in less than 15 minutes after sample preparation and lysis.
3. Simple cleavage with proteinase K without any mechanical
cleavage.
4. Minimal RNA contamination.
5. Purified genomic DNA exhibits improved downstream performance in
applications such as PCR.
6. A kit is included for automated processing of large numbers of
samples in 96-well plates using a liquid handling robot.
model | CY-F006-10 (50 preps-cells) |
Reagent capacity 1 | CY1: 30mL |
Reagent capacity 2 | CY2: 30mL |
Reagent capacity 3 | CY3: 12mL |
Reagent capacity 4 | CY4: 18mL |
Reagent capacity 5 | CY5: 6mL |
Product Usage:
For purification and isolation of DNA (including genomic,
mitochondrial, bacterial, parasitic and viral DNA) from tissues,
saliva, body fluids and oral cavity, cervix, skin cells, bacterial
cells, tissue, swabs, cerebrospinal fluid, body fluids, washed
urine cells )
Product Instructions:
The magnetic bead material specifically adsorbs and dissociates
from nucleic acid under different salt concentrations and acid-base
conditions, separates nucleic acid and other components in the
lysed cells by the transfer magnetic bead method, and then obtains
the magnetic bead-free extract by washing and elution- -- Nucleic
acids.
The first step - lysis, use the lysis solution to destroy the cellular structure of animal and plant cells, so that the DNA is fully released.
The second step—binding, adding magnetic bead binding solution to specifically adsorb DNA, so that DNA can be fully combined with magnetic beads;
The third step------washing, using detergent, after multiple washings, fully remove other residual impurities;
The fourth step - elution, use the eluent to dissociate the magnetic beads and DNA to obtain a higher-quality DNA solution.