Long Taq Mix PCR Master Kit #P2061 1ml

Long Taq Mix

#P2061, 1 ml

Contents:

Store at -20°C

For research use only.

In total 3 vials.

Description

2× Long Taq Mix is a premixed, ready-to-use solution containing Taq DNA Polymerase, dNTPs, Mg²⁺ and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR. To prepare the final PCR, only need to add primers and template DNA. This premixed formulation saves time and reduces contamination due to the fewer pipetting steps required for PCR set-up. It also contributes to higher sensitivity by adding enhancer.

Long Taq DNA Polymerase, a combination of two thermostable DNA polymerases, Taq and Pfu, is a special formulation designed for amplifying large fragments. This specially formulated Long Taq was shown to amplify long templates from λ phage genome that up to 20 kb. The elongation rate is 3kb/min. It is also a better choice for amplifying complex template, such as GC-rich template. Long Taq is suitable as a direct replacement for ordinary Taq Polymerase in most applications. Using Long Taq in your PCR reactions results in 3´-dA overhangs PCR products, which can be used in TA clone.

Composition of the 2× Long Taq Mix

0.25U/μl Taq DNA Polymerase, 0.01U/μl Pfu DNA Polymerase, 2× PCR Buffer, 0.4mM dNTPs, 3.2mM MgCl_2, 0.02% bromophenol blue.

Long Taq Mix buffer is a proprietary formulation optimized for robust performance in PCR.

Applications

• PCR for long templates of up to 20 kb

• High reproducible, high throughput PCR for complex template

Features

• Convenient: only primers and template are needed to add when prepare PCR system

• Longer fragment: amplify long templates as long as 20 kb

• High efficiency: saving your time by simplifying the process

• Reproducible: lower contamination and pipetting error risk

• Amplification of complex template (GC-rich or repetitive sequence)

Dongsheng Biotech Co., Ltd takes PCR technology as its core, and its products focus on common PCR, qPCR, nucleic acid detection and other fields. Adhering to the quality policy of "unremitting pursuit of continuous innovation, leading technology and customer satisfaction", we provide our customers with cost-effective molecular biology products.