Taq Mix PCR Master Kit #P2012 5ml

Taq Mix

#P2012, 5 ml

Contents:

Store at -20°C

For research use only.

In total 10 vials.

Description

2× Taq Mix is a premixed, ready-to-use solution containing Taq DNA Polymerase, dNTPs, Mg²⁺ and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR. To prepare the final PCR, only need to add primers and template DNA. This premixed formulation saves time and reduces contamination due to the fewer pipetting steps required for PCR set-up. The mix retains all features of Taq DNA Polymerase.

Taq DNA Polymerase is a thermostable recombinant DNA polymerase derived from thermophilic bacterium Thermus aquaticus. Its molecular weight is 94 kDa. Taq DNA Polymerase can amplify DNA target up to 5 kb (simple template). The elongation velocity is 2kb/min (70~75°C). It has 5' to 3' polymerase activity but lacks of 3' to 5' exonuclease activity that results in a 3'-dA overhangs PCR product.

Composition of the 2× Taq Mix

0.25U/ul Taq DNA Polymerase, 2× PCR Buffer, 0.4mM dNTPs, 3.2mM MgCl_2, 0.02% bromophenol blue.

Taq Mix buffer is a proprietary formulation optimized for robust performance in PCR.

Applications

• High throughput PCR

• Routine PCR with high reproducibility

• Generation of PCR products for TA cloning

Features

• Convenient: only primers and template DNA are added

when prepare final PCR

• High yields of PCR products with minimal optimization

• High efficiency: saving your time by simplifying the process

• Reproducible: lower contamination and pipetting error risk

Dongsheng Biotech Co., Ltd takes PCR technology as its core, and its products focus on common PCR, qPCR, nucleic acid detection and other fields. Adhering to the quality policy of "unremitting pursuit of continuous innovation, leading technology and customer satisfaction", we provide our customers with cost-effective molecular biology products.